Construct: ORF TRCN0000479957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008149.1_s317c1
- Derived from:
- ccsbBroadEn_04092
- DNA Barcode:
- GTTTCGGCGAGGAGTAGTGCCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFAIP8L2 (79626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79626 | TNFAIP8L2 | TNF alpha induced protein 8... | NM_024575.5 | 100% | 100% | |
| 2 | mouse | 69769 | Tnfaip8l2 | tumor necrosis factor, alph... | NM_027206.2 | 86.2% | 94% | (many diffs) |
| 3 | mouse | 69769 | Tnfaip8l2 | tumor necrosis factor, alph... | XM_006502040.3 | 86.2% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 621
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtccttc agctcaaaga gcctggcact gcaagcagag aagaagctac 121 tgagtaagat ggcgggtcgc tctgtggctc atctcttcat agatgagaca agcagtgagg 181 tgctagatga gctctaccgt gtgtccaagg agtacacgca cagccggccc caggcccagc 241 gcgtgatcaa ggaccTGATC AAAGTGGCCA TCAAGGTGGC TGTGCTGCAC CGCAATGGCT 301 CCTTTGGCCC CAGTGAGCTG GCCCTGGCTA CCCGCTTTCG CCAGAAGCTG CGGCAGGGTG 361 CCATGACGGC ACTTAGCTTT GGTGAGGTAG ACTTCACCTT CGAGGCTGCT GTTCTGGCTG 421 GCCTGCTGAC CGAGTGCCGG GATGTGCTGC TAGAGTTGGT GGAACACCAC CTCACGCCCA 481 AGTCACATGG CCGCATCCGC CACGTGTTTG ATCACTTCTC TGACCCAGGT CTGCTCACGG 541 CCCTCTATGG GCCTGACTTC ACTCAGCACC TTGGCAAGAT CTGTGACGGA CTCAGGAAGC 601 TGCTAGACGA AGGGAAGCTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTT CGGCGAGGAG 781 TAGTGCCAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt