Construct: ORF TRCN0000480067
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000967.1_s317c1
- Derived from:
- ccsbBroadEn_06285
- DNA Barcode:
- TTGTAGGAGTCTTAGTCTTACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLUD1 (2746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480067
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_005271.5 | 99.9% | 99.8% | 1663G>C |
| 2 | human | 2747 | GLUD2 | glutamate dehydrogenase 2 | NM_012084.4 | 97.5% | 95.5% | (many diffs) |
| 3 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318900.1 | 75.1% | 72.4% | (many diffs) |
| 4 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318901.1 | 70% | 69.8% | 0_1ins501;1162G>C |
| 5 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318902.1 | 70% | 69.8% | 0_1ins501;1162G>C |
| 6 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318904.2 | 70% | 69.8% | 0_1ins501;1162G>C |
| 7 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318905.2 | 70% | 69.8% | 0_1ins501;1162G>C |
| 8 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318906.2 | 70% | 69.8% | 0_1ins501;1162G>C |
| 9 | mouse | 14661 | Glud1 | glutamate dehydrogenase 1 | NM_008133.4 | 92.2% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ccgctacctg ggcgaagcgc tgttgctgtc ccgggccggg cccgctgccc 121 tgggctcggc gtccgccgac tcggccgcgt tgctgggctg ggcccgggga cagcccgccg 181 ccgccccgca gccggggctg gcattggccg cccggcgcca ctacagcgag gcggtggccg 241 accgcgagga cgaccccaac ttcttcaaga tggtggaggg cttcttcgat cgcggcgcca 301 gcatcgtgga ggacaagctg gtggaggacc tgaggacccg ggagagcgag gagcagaagc 361 ggaaccgggt gcgcggcatc ctgcggatca tcaagccctg caaccatgtg ctgagtctct 421 ccttccccat ccggcgcgac gacggctcct gggaggtcat cgaaggctac cgggcccagc 481 acagccagca ccgcacgccc tgcaagggag gtatccgtta cagcactgat gtgagtgtag 541 atgaagtaaa agctttggct tctctgatga catacaagtg tgcagtggtt gatgtgccgt 601 ttgggggtgc taaagctggt gttaagatca atcccaagaa ctatactgat aatgaattgg 661 aaaagatcac aaggaggttc accatggagc tagcaaaaaa gggctttatt ggtcctggca 721 ttgatgtgcc tgctccagac atgagcacag gtgagcggga gatgtcctgg atcgctgata 781 cctatgccag caccataggg cactatgata ttaatgcaca cgcctgtgtt actggtaaac 841 ccatcagcca agggggaatc catggacgca tctctgctac tggccgtggt gtcttccatg 901 ggattgaaaa tttcatcaat gaagcttctt acatgagcat tttaggaatg acaccagggt 961 ttggagataa aacatttgtt gttcagggat ttggtaatgt gggcctacac tctatgagat 1021 atttacatcg ttttggtgct aaatgtattg ctgttggtga gtctgatggg agtatatgga 1081 atccagatgg tattgaccca aaggaactgg aagacttcaa attgcaacat gggtccattc 1141 tgggcttccc caaggcaaag ccctatgaag gaagcatctt ggaggccgac tgtgacatac 1201 tgatcccagc tgccagtgag aagcagttga ccaaatccaa cgcacccaga gtcaaagcca 1261 agatcattgc tgaaggtgcc aatgggccaa caactccaga agctgacaag atcttcctgg 1321 agagaaacat tatggttatt ccagatctct acttgaatgc tggaggagtg acagtatctt 1381 actttGAGTG GCTGAAGAAT CTAAATCATG TCAGCTATGG CCGTTTGACC TTCAAATATG 1441 AAAGGGATTC TAACTACCAC TTGCTCATGT CTGTTCAAGA GAGTTTAGAA AGAAAATTTG 1501 GAAAGCATGG TGGAACTATT CCCATTGTAC CCACGGCAGA GTTCCAAGAC AGGATATCGG 1561 GTGCATCTGA GAAAGACATC GTGCACTCTG GCTTGGCATA CACAATGGAG CGTTCTGCCA 1621 GGCAAATTAT GCGCACAGCC ATGAAGTATA ACCTGGGATT GGACCTGAGA ACAGCTGCCT 1681 ATGTTAATGC CATTGAGAAA GTCTTCAAAG TGTACAATGA AGCTGGTCTG ACCTTCACAT 1741 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1801 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1861 TTTATATATC TTGTGGAAAG GACGATTGTA GGAGTCTTAG TCTTACGTGA CGCGTTAAGT 1921 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt