Construct: ORF TRCN0000480111
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000659.1_s317c1
- Derived from:
- ccsbBroadEn_07514
- DNA Barcode:
- CCCACGGCCATCGGGTTACAAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAFB (9935)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480111
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9935 | MAFB | MAF bZIP transcription fact... | NM_005461.5 | 99.8% | 100% | 639C>T |
| 2 | mouse | 16658 | Mafb | v-maf musculoaponeurotic fi... | NM_010658.3 | 92.9% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcggagctg agcatggggc cagagctgcc caccagcccg ctggccatgg 121 agtatgtcaa cgacttcgac ctgctcaagt tcgacgtgaa gaaggagcca ctggggcgcg 181 cggagcgtcc gggcaggccc tgcacacgcc tgcagccagc cggctcggtg tcctccacac 241 cgctcagcac tccgtgtagc tccgtgccct cgtcgcccag cttcagcccg accgaacaga 301 agacacacct cgaggatctg tactggatgg cgagcaacta ccagcagatg aaccccgagg 361 cgctcaacct gacgcccgag gacgcggtgg aagcgctcat cggctcgcac ccagtgccac 421 agccgctgca aagcttcgac agctttcgcg gcgctcacca ccaccaccat caccaccacc 481 ctcacccgca ccacgcgtac ccgggcgccg gcgtggccca cgacgagctg ggcccgcacg 541 ctcacccgca ccatcaccat catcaccaag cgtcgccgcc gccgtccagc gccgctagcc 601 cggcgcaaca gctgcccact agccaccccg ggcccgggcc gcacgcgacg gcctcggcga 661 cggcggcggg cggcaacggc agcgtggagg accgcttcTC CGATGACCAG CTCGTGTCCA 721 TGTCCGTGCG CGAGCTGAAC CGCCACCTGC GGGGCTTCAC CAAGGACGAG GTGATCCGCC 781 TGAAGCAGAA GCGGCGGACC CTGAAGAACC GGGGCTACGC CCAGTCTTGC AGGTATAAAC 841 GCGTCCAGCA GAAGCACCAC CTGGAGAATG AGAAGACGCA GCTCATTCAG CAGGTGGAGC 901 AGCTTAAGCA GGAGGTGTCC CGGCTGGCCC GCGAGAGAGA CGCCTACAAG GTCAAGTGCG 961 AGAAACTCGC CAACTCCGGC TTCAGGGAGG CGGGCTCCAC CAGCGACAGC CCCTCCTCTC 1021 CCGAGTTCTT TCTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCCACGGCCA TCGGGTTACA 1201 AACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt