Construct: ORF TRCN0000480114

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018064.1_s317c1
Derived from:
ccsbBroadEn_15381
DNA Barcode:
AACTATTATCAGGCAGATCCTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDKN1A (1026)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480114
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1026 CDKN1A cyclin dependent kinase inh... NM_000389.5 99.7% 99.3% 93C>A
2 human 1026 CDKN1A cyclin dependent kinase inh... NM_001220777.1 99.7% 99.3% 93C>A
3 human 1026 CDKN1A cyclin dependent kinase inh... NM_001220778.1 99.7% 99.3% 93C>A
4 human 1026 CDKN1A cyclin dependent kinase inh... NM_078467.2 99.7% 99.3% 93C>A
5 human 1026 CDKN1A cyclin dependent kinase inh... NM_001291549.2 82.6% 82.3% 1_102del;195C>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
558
ORF length:
492
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc agaaccggct ggggatgtcc gtcagaaccc atgcggcagc aaggcctgcc
121gccgcctctt cggcccagtg gacagcgagc agctgagacg cgactgtgat gcgctaatGG
181CGGGCTGCAT CCAGGAGGCC CGTGAGCGAT GGAACTTCGA CTTTGTCACC GAGACACCAC
241TGGAGGGTGA CTTCGCCTGG GAGCGTGTGC GGGGCCTTGG CCTGCCCAAG CTCTACCTTC
301CCACGGGGCC CCGGCGAGGC CGGGATGAGT TGGGAGGAGG CAGGCGGCCT GGCACCTCAC
361CTGCTCTGCT GCAGGGGACA GCAGAGGAAG ACCATGTGGA CCTGTCACTG TCTTGTACCC
421TTGTGCCTCG CTCAGGGGAG CAGGCTGAAG GGTCCCCAGG TGGACCTGGA GACTCTCAGG
481GTCGAAAACG GCGGCAGACC AGCATGACAG ATTTCTACCA CTCCAAACGC CGGCTGATCT
541TCTCCAAGAG GAAGCCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
601TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
661GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACTATT ATCAGGCAGA
721TCCTTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)