Construct: ORF TRCN0000480165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000566.1_s317c1
- Derived from:
- ccsbBroadEn_12209
- DNA Barcode:
- AACCAGTCATGTCCCGTTGACGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAPTM4B (55353)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55353 | LAPTM4B | lysosomal protein transmemb... | NM_018407.6 | 100% | 100% | |
| 2 | mouse | 114128 | Laptm4b | lysosomal-associated protei... | NM_033521.3 | 88.2% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatggtcgcg ccctggacgc ggttctactc caacagctgc tgcttgtgct 121 gccatgtccg caccggcacc atcctgctcg gcgtctggta tctgatcatc aatgctgtgg 181 tactgttgat tttattgagt gccctggctg atccggatca gtataacttt tcaagttctg 241 aactgggagg tgactttgag ttcatggatg atgccaacat gtgcattgcc attgcgattt 301 ctcttctcat gatccTGATA TGTGCTATGG CTACTTACGG AGCGTACAAG CAACGCGCAG 361 CCTGGATCAT CCCATTCTTC TGTTACCAGA TCTTTGACTT TGCCCTGAAC ATGTTGGTTG 421 CAATCACTGT GCTTATTTAT CCAAACTCCA TTCAGGAATA CATACGGCAA CTGCCTCCTA 481 ATTTTCCCTA CAGAGATGAT GTCATGTCAG TGAATCCTAC CTGTTTGGTC CTTATTATTC 541 TTCTGTTTAT TAGCATTATC TTGACTTTTA AGGGTTACTT GATTAGCTGT GTTTGGAACT 601 GCTACCGATA CATCAATGGT AGGAACTCCT CTGATGTCCT GGTTTATGTT ACCAGCAATG 661 ACACTACGGT GCTGCTACCC CCGTATGATG ATGCCACTGT GAATGGTGCT GCCAAGGAGC 721 CACCGCCACC TTACGTGTCT GCCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACCAGTCATG 901 TCCCGTTGAC GTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att