Construct: ORF TRCN0000480170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018258.2_s317c1
- Derived from:
- ccsbBroadEn_11883
- DNA Barcode:
- AGAAGAAATCTGTTAACTGCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGLV3-25 (28793)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 54.1% | 48.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggatccct ctacttctcc ccctcctcac tctctgcaca ggctctgagg 121 cctcctatga gctgacacag ccaccctcgg tgtcagtgtc cccaggacag acggccagga 181 tcacctgctc tggagatgca ttgccaaagc aatatgctta ttggtaccag cagaagccag 241 gccaggcccc tgtgttggtg atatataaag acaatgagag gccctcaggg atccctgagc 301 gattctctgg ctccagctca gggacaacag tcacgttgac catcagtgga gtccaggcag 361 aagacgaggc tgactattac tgtcaatcag cagacagcag tggtacttat tgggtgttcg 421 gcggagggac caagctgacc gtcctaggtc agcccaaggc tgccccctcg gtcactctgt 481 tcccgccctc cTCTGAGGAG CTTCAAGCCA ACAAGGCCAC ACTGGTGTGT CTCATAAGTG 541 ACTTCTACCC GGGAGCCGTG ACAGTGGCCT GGAAGGCAGA TAGCAGCCCC GTCAAGGCGG 601 GAGTGGAGAC CACCACACCC TCCAAACAAA GCAACAACAA GTACGCGGCC AGCAGCTACC 661 TGAGCCTGAC GCCTGAGCAG TGGAAGTCCC ACAAAAGCTA CAGCTGCCAG GTCACGCATG 721 AAGGGAGCAC CGTGGAGAAG ACAGTGGCCC CTACAGAATG TTCATACCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA AGAAGAAATC TGTTAACTGC TTCAACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt