Construct: ORF TRCN0000480186
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008114.1_s317c1
- Derived from:
- ccsbBroadEn_03791
- DNA Barcode:
- AAGTCGATTATACGCGGGAATGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAN1C1 (57134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480186
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57134 | MAN1C1 | mannosidase alpha class 1C ... | NM_020379.3 | 100% | 100% | |
| 2 | human | 57134 | MAN1C1 | mannosidase alpha class 1C ... | NM_001289010.1 | 93.5% | 93.4% | 1768_1769delATinsTA;1770_1771ins120 |
| 3 | human | 57134 | MAN1C1 | mannosidase alpha class 1C ... | XM_017001860.1 | 61.4% | 60% | 0_1ins613;23_24ins116 |
| 4 | human | 57134 | MAN1C1 | mannosidase alpha class 1C ... | XM_017001861.1 | 46.2% | 35.2% | (many diffs) |
| 5 | human | 57134 | MAN1C1 | mannosidase alpha class 1C ... | XR_001737333.1 | 37.4% | 1_268del;1744_1745ins173;1986_4406del | |
| 6 | human | 57134 | MAN1C1 | mannosidase alpha class 1C ... | XR_001737334.1 | 36.4% | 1_272del;1527_1528ins220;1943_4363del | |
| 7 | mouse | 230815 | Man1c1 | mannosidase, alpha, class 1... | NM_207237.3 | 84.2% | 88.2% | (many diffs) |
| 8 | mouse | 230815 | Man1c1 | mannosidase, alpha, class 1... | XM_011250232.2 | 69.3% | 65.1% | (many diffs) |
| 9 | mouse | 230815 | Man1c1 | mannosidase, alpha, class 1... | XM_006538757.3 | 62.5% | 64.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1959
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcatgagg aaagtgcccg gcttcgtccc ggcctccccg tgggggctgc 121 ggctgccgca gaagttcctc ttcctcctct tcctctcggg cctggtcacc ctgtgcttcg 181 gggccctctt cctgctgccc cactcctctc gcctcaagcg cctcttcctg gccccccgga 241 cccagcagcc tggtctggaa gtggtggctg aaatcgccgg ccatgccccg gcccgcgagc 301 aggagccgcc tcccaacccg gcccccgccg cgccggcccc gggcgaggat gaccccagca 361 gctgggccag tccccgccgc aggaaagggg ggctgcggcg cacccgcccc actggacccc 421 gcgaggaggc cacggcggcc cggggcaata gcatcccggc ctccaggccc ggggacgagg 481 gcgtcccttt ccgctttgac ttcaacgcat tccggagccg tctccgccac ccggtcctgg 541 gaacgagggc cgatgagagt caggagcccc agagccaagt gcgagcccag cgggagaaaa 601 tcaaggagat gatgcagttt gcttggcaga gctataagcg ttatgcaatg gggaaaaacg 661 aactccgtcc actaacaaaa gatggctacg agggtaacat gttcggaggc ctcagcgggg 721 caacagtcat tgactccctc gataccctct acctcatgga gctgaaggag gagttccagg 781 aggccaaggc ctgggtggga gagagcttcc acctgaacgt gagcggagaa gcatccttgt 841 ttgaggtgaa catccgctac atcgggggac tcctctcagc cttctacctg acaggagaag 901 aggtgttccg aataaaggcc atcaggctgg gagagaagct cctgccggcg ttcaacaccc 961 ccacgggaat cccaaagggc gtggtgagct tcaaaagtgg gaactggggc tgggccacag 1021 ccggcagcag cagcatcttg gcggagtttg gatccctgca cttggaattc ttacacctca 1081 ctgaactctc tggcaaccag gtcttcgctg aaaaggtcag gaacatccgc aaggtcctca 1141 ggaagatcga aaagcccttt ggcctctacc ccaacttcct cagcccagtg agtgggaact 1201 gggtgcaaca ccatgtctca gttggaggac tcggggacag tttttatgaa tatttgatca 1261 aatcctggtt gatgtcgggc aagacagata tggaggctaa aaatatgtac tacgaagcct 1321 tggaggcgat agagacctac ttgctgaatg tctctcccgg ggggctgacc tacattgccg 1381 agtggcgagg ggggattctg gaccacaaga tggggcacct ggcctgtttc tccgggggca 1441 tgatcgccct tggcgccgag gatgccaagg aagaaaagag ggcccactac cgagagctcg 1501 cagcccagat caccaagacg tgtcacgagt catacgcccg ctcagacacc aaacttgggc 1561 ctgaggcctt ctggtttaac tccggcagag aggccgtggc cacccagctg agcgagagct 1621 actacatccT CCGGCCAGAG GTGGTGGAGA GCTACATGTA CCTGTGGCGA CAGACCCACA 1681 ACCCCATCTA CAGGGAGTGG GGCTGGGAGG TGGTGCTGGC CTTGGAGAAA TACTGTCGGA 1741 CAGAAGCCGG TTTCTCTGGG ATCCAAGACG TGTACAGTAG CACCCCCAAC CACGACAACA 1801 AGCAGCAGAG CTTCTTTCTA GCGGAGACAC TAAAGTATCT CTATCTTCTG TTCTCTGAAG 1861 ATGACTTGCT CTCCCTGGAA GACTGGGTGT TCAACACCGA GGCCCACCCA CTCCCGGTGA 1921 ACCACTCAGA CAGCTCCGGC AGAGCCTGGG GCAGACACTT GCCAACTTTC TTGTACAAAG 1981 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2041 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2101 ACGAAAGTCG ATTATACGCG GGAATGCGAC GCGTTAAGTC gacaatcaac ctctggatta 2161 caaaatttgt gaaagatt