Construct: ORF TRCN0000480243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007699.1_s317c1
- Derived from:
- ccsbBroadEn_08550
- DNA Barcode:
- TAGAAAGACCCTTCCTTCTACCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF280C (55609)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55609 | ZNF280C | zinc finger protein 280C | NM_017666.5 | 99.9% | 100% | 1851A>C |
| 2 | human | 55609 | ZNF280C | zinc finger protein 280C | XM_006724765.3 | 93.3% | 93.3% | 1245_1246ins147;1704A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2277
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacgacaaa ccttttcaac caaaaaacat ttcaaaaatg gcagaactct 121 ttatggaatg tgaagaagaa gagctagagc catggcagaa gaaagtagaa gaaactcagg 181 atgaggatga cgatgaactg atctttgttg gagagatatc aagttcaaaa ccagccattt 241 caaatatttt gaacagaggc cactccagct catcttcaaa aggaataaag agtgagccac 301 acagtccagg tattcctgaa atattcagga ctgcaagtca acgctgcaga gacccaccat 361 caaatccagt ggctgcctcg cctagatttc atcttgtatc taaatcttca caaagctctg 421 ttactgttga gaatgcgtct aaacctgatt ttacaaagaa ttcacaagtt ggatcggata 481 attcttcaat tttactgttt gactcgaccc aggaatcact accaccatcc caagacatac 541 cagcaatttt tagagaaggt atgaaaaata cttcatatgt gttgaaacat ccttctactt 601 ctaaagtaaa cagtgttact ccaaaaaaac caaagaccag tgaagatgtt cctcagataa 661 atccctccac ttcattgcct ttaattggct ctcctccagt gacatcctcc caagttatgc 721 tgtcaaaagg tacaaatacc tcatctccat atgatgctgg agcagattac ctaagagctt 781 gtccaaagtg caatgttcag ttcaatcttt tggatccttt gaaataccac atgaagcatt 841 gttgtccaga catgataact aaatttttgg gagtaattgt taaatcagaa cgtccatgtg 901 atgaagacaa gactgattca gagacaggaa agttgatcat gttagtcaat gagttttatt 961 atggaaggca tgaaggagtc actgagaaag agccaaagac ttacacaacc tttaaatgct 1021 tcagttgctc gaaagttctt aaaaataata ttaggtttat gaaccacatg aaacatcact 1081 tggaacttga gaagcagaac aatgaaagct gggaaaacca caccacctgc cagcactgtt 1141 accggcaata tcccacaccc ttccaactgc agtgccacat tgagagtaca cacactcccc 1201 atgagttttc tactatttgc aaaatctgtg aattatcatt tgaaacagag catattcttt 1261 tacaacatat gaaggacacc cataaacctg gtgaaatgcc atatgtttgc caggtttgcc 1321 agtttagatc atcaacattt tctgatgtag aagctcattt tagagcagcc catgaaaaca 1381 ctaagaactt gctatgtcca ttttgcctca aagttagtaa aatggcaacc ccctacatga 1441 atcattacat gaagcatcag aaaaaaggag ttcatcgttg cccaaaatgc agactacaat 1501 ttttgaccag caaggagaaa gctgaacata aggcgcagca tcgtacattt ataaagccta 1561 aagaactaga aggattgcct cctggagcaa aagttactat tcgagcttca cttggacctc 1621 tccagtcaaa attaccaact gcacctttcg gttgcgctcc aggcacttct tttcttcagg 1681 tcacacctcc gacatctcaa aatacaactg ctagaaatcc tagaaaatct aatgccagta 1741 gatctaagac aagtaagctt catgcaacta catccactgc aagtaaagtt aatacaagta 1801 agccaagggg acgtatagct aagtccaaag caaaaccctc ttacaagcaa aagcgacagc 1861 gcaacagaaa aaataaaatg agccttgctt tgaagaacat aaggtgtcgt cggggcattc 1921 acaagtgcat tgaatgtcat tccaaaataa aagattttgc aagccacttt tctatataca 1981 TCCACTGCAG TTTTTGCAAG TACAATACTA ACTGTAACAA AGCCTTTGTA AATCATATGA 2041 TGAGCTCTCA TAGCAACCAT CCAGGTAAAC GGTTTTGTAT TTTCAAGAAG CATTCAGGAA 2101 CTCTCAGGGG CATTACTCTA GTGTGCCTTA AATGTGATTT CCTAGCTGAT TCTTCCGGCT 2161 TAGATCGTAT GGCTAAACAC TTAAGTCAAC GTAAAACTCA TACTTGCCAA GTTATAATAG 2221 AGAATGTTTC CAAAAGTACC TCAACTTCTG AACCCACTAC TGGCTGCTCA TTGAAATACC 2281 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GATAGAAAGA CCCTTCCTTC TACCCAACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt