Construct: ORF TRCN0000480273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006745.1_s317c1
- Derived from:
- ccsbBroadEn_10175
- DNA Barcode:
- ATGTTCCTGTTCAAGACCTTCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AB1 (474382)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 474382 | H2AB1 | H2A.B variant histone 1 | NM_001017990.1 | 99.7% | 99.1% | 325C>A |
| 2 | human | 474381 | H2AB2 | H2A.B variant histone 2 | NM_001017991.2 | 99.4% | 98.2% | 203T>C;325C>A |
| 3 | human | 83740 | H2AB3 | H2A.B variant histone 3 | NM_080720.1 | 99.4% | 98.2% | 203T>C;325C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 427
- ORF length:
- 345
- Sequence:
-
1 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT 61 TTGTACAAAA AAGTTGGCAC CATGCCGAGG AGGAGGAGAC GCCGAGGGTC CTCCGGTGCT 121 GGCGGCCGGG GGCGGACCTG CTCTCGCACC GTCCGAGCGG AGCTTTCGTT TTCAGTGAGC 181 CAGGTGGAGC GCAGTCTACG GGAGGGCCAC TACGCTCAGC GCCTGAGTCG CACGGCGCCG 241 GTCTACCTCG CTGCGGTTAT TGAGTACCTG ACGGCCAAGG TCCCGGAGCT GGCGGGCAAC 301 GAGGCCCAGA ACAGCGGAGA GCGGAACATC ACTCCCCTGC TGCTGGACAT GGTGGTTCAC 361 AACGACAGGC TACTGAGCAC CCTTTTCAAC ACGACCACCA TCTCTAAAGT GGCCCCTGGC 421 GAGGACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT 481 CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT 541 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGTTCCT GTTCAAGACC TTCACAACGC 601 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt