Construct: ORF TRCN0000480282
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018252.1_s317c1
- Derived from:
- ccsbBroadEn_01829
- DNA Barcode:
- CGTTGTAAGCCTCCGCCCTTTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BSND (7809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480282
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7809 | BSND | barttin CLCNK type accessor... | NM_057176.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgacgag aagaccttcc ggatcggctt cattgtgctg gggcttttcc 121 tgctggccct cggtacgttc ctcatgagcc atgatcggcc ccaggtctac ggcaccttct 181 atgccatggg cagcgtcatg gtgatcgggg gcatcatctg gagcatgtgc cagtgctacc 241 ccaagatcac cttcgtccct gctgactctg actttcaagg catcctctcc ccaaaggcca 301 tgggcctgct ggagaatggg cttgctgccg agatgaagag ccccagtccc cagccgccct 361 atgtaaggct gtgggaggaa gccgcctatg accagagcct gcctgacttc agccacatcc 421 agatgaaagt catgagctac agtgaggacc accgctcctt gctggcccct gagatggggc 481 agccgaagct gggaaccagt gatggaggag aaggtggccc tggcgacgtt caggcctgga 541 tggaggctgc cgtggtcatc cacaagggct cagacgagag tgaaggggaa agacgcctaa 601 ctcagagctg gcccggcccc ctggcctgtc cccagggccc tgcccccttg gcttccttcc 661 aagatgacct ggacatggac tccagtgaag gcagcagccc caatgcatct ccacatgaca 721 gggaggaagc ttgttcccca caacaggaac cTCAGGGCTG CAGGTGCCCG CTGGACCGCT 781 TCCAAGACTT TGCCCTGATT GATGCCCCAA CGTTGGAGGA TGAGCCCCAA GAGGGGCAGC 841 AGTGGGAAAT AGCCCTGCCC AACAACTGGC AGCGGTACCC AAGGACAAAG GTGGAGGAGA 901 AGGAGGCTTC GGACACAGGT GGGGAGGAAC CTGAGAAGGA AGAGGAAGAC CTGTACTATG 961 GGCTGCCAGA TGGAGCCGGG GACCTCCTCC CGGACAAGGA GCTGGGTTTT GAGCCTGACA 1021 CCCAAGGCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTTGT AAGCCTCCGC CCTTTGACAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt