Construct: ORF TRCN0000480290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013621.1_s317c1
- Derived from:
- ccsbBroadEn_05717
- DNA Barcode:
- AGCTAAACTGGTTTAAGAGCGCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHAF1 (644096)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 644096 | SDHAF1 | succinate dehydrogenase com... | NM_001042631.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 414
- ORF length:
- 345
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagccggcac agccggctgc agaggcaggt tctgagcctg taccgcgatc 121 tgctgcgcgc cgggcgtggg aagccgggcg ccgaggcgcG AGTGCGGGCA GAGTTCCGGC 181 AGCATGCGGG CCTGCCGCGG TCCGACGTGC TGCGCATCGA GTACCTGTAC CGCCGCGGGC 241 GGCGCCAGCT GCAGCTGCTA CGCTCGGGCC ACGCCACCGC CATGGGCGCC TTCGTACGCC 301 CGCGGGCCCC GACCGGGGAG CCTGGCGGCG TGGGTTCCCA GCCTGACGAC GGCGACAGTC 361 CAAGGAACCC CCACGACAGC ACGGGGGCAC CGGAGACCCG CCCCGACGGA CGGTTGCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAA GCTAAACTGG TTTAAGAGCG CATACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att