Construct: ORF TRCN0000480297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009505.1_s317c1
- Derived from:
- ccsbBroadEn_12625
- DNA Barcode:
- ATTGACCACTTCAACCCGGATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCC1 (79825)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480297
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79825 | EFCC1 | EF-hand and coiled-coil dom... | XM_011513164.2 | 53.1% | 53.1% | 1_426del |
| 2 | human | 79825 | EFCC1 | EF-hand and coiled-coil dom... | XM_011513161.2 | 43.5% | 43.5% | 1_627del |
| 3 | human | 79825 | EFCC1 | EF-hand and coiled-coil dom... | NM_024768.2 | 26.9% | 26.9% | 1_1311del |
| 4 | human | 79825 | EFCC1 | EF-hand and coiled-coil dom... | XM_005247781.4 | 26.8% | 26.8% | 1_1314del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 552
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacttacttt ggtcacttcg gcggtgccaa ccatgcccat accctggggg 121 agctggaggc ctgcattgcc atgctggtgg agcagctgag gactcagggc tgcggtggga 181 ggaccctggg gaccTCTGAG GAGGAGGCAG AGTTGCAGCA GAAGGTGGAA GAGAATGAGC 241 ACCTGAGGCT GGAGCTGCAG ATGGTAGAGA CCGAGAGGGT GCGGCTGTCC CTGCTGGAGG 301 AGAAGCTGGT GGACGTGCTG CAGCTCCTGC AGAGGCTCCG GGACCTGAAC ATATCGAAAA 361 GAGCCCTGGG GAAGATTTTG CTGAGCACGC TGGACGCTTT CAGGGACCCC ACCCACGAGG 421 GAAGGCCCAG CCCTGCAGCC ATCCTGGATG CCCTGCACCA AGCCTTGGCT GCCTGCCAGC 481 TGTTGCGGAG ACAGCCCTCG GCACCAGCCT CTGCAGCAGC TGCGCTCACC AACCCCCTCC 541 TCGTCTCCTG CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATT GACCACTTCA ACCCGGATAT 721 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t