Construct: ORF TRCN0000480342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015865.1_s317c1
- Derived from:
- ccsbBroadEn_13215
- DNA Barcode:
- ACATTCCCACGGCCTACGTTGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACUL1 (143384)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480342
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143384 | CACUL1 | CDK2 associated cullin doma... | NM_153810.5 | 81.3% | 79.9% | (many diffs) |
| 2 | mouse | 78832 | Cacul1 | CDK2 associated, cullin dom... | NM_001172097.1 | 78% | 79.5% | (many diffs) |
| 3 | mouse | 78832 | Cacul1 | CDK2 associated, cullin dom... | NM_001172096.1 | 74.5% | 70.3% | (many diffs) |
| 4 | mouse | 78832 | Cacul1 | CDK2 associated, cullin dom... | NM_030197.2 | 72.7% | 73.4% | (many diffs) |
| 5 | mouse | 78832 | Cacul1 | CDK2 associated, cullin dom... | XM_006527468.3 | 45.8% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaaagcatg gaagaggagg aggggggcag ctacgaggcg atgatggacg 121 accagaacca caacaactgg gaggctgcgg tggacggctt ccggcagccc ctgccacctc 181 cgccgccccc ctcgtcgatc ccggcccctg cccgagagcc tccggggggg cagctgctgg 241 cggtgcccgc ggtctccgtg gacaggaaag gccccaagga ggggctcccg atggggccgc 301 agccaccgcc ggaggctaat ggggtgatca tgatgttgaa gagctgcgac gcggccgccg 361 ccgtggccaa ggcggccccc gcccccaccg ccagctccac catcaacatc aacacctcca 421 cctccaagtt cttaatgaat gttataacta ttgaagatta taagagcaca tactggccaa 481 aattggatgg tgccatagat caacttttaa ctcagagtcc TGGTGACTAT ATCCCCATAT 541 CCTATGAACA GATATACAGT TGTGTGTATA AATGTGTATG CCAGCAGCAC TCGGAACAGA 601 TGTATAGTGA TCTGATTAAA AAGATAACTA ATCACTTAGA GAGAGTCTCA AAGGAGCTGC 661 AGGCCAGCCC TCCAGATCTC TATATTGAAA GATTTAATAT AGCTCTTGGA CAATATATGG 721 GAGCATTGCA GAGCATTGTG CCTCTTTTCA TATATATGAA TAAGTTTTAC ATCGAAACCA 781 AGCTTAACAG AGACTTAAAA GATGACCTTA TAAAGCTGTT TACGGAACAT GTTGCAGAAA 841 AGCACATTTA CAGCCTAATG CCTTTACTTT TAGAAGCCCA GTCAACACCA TTTCAGGTCA 901 CACCTTCAAC TATGGCAAAT ATTGTGAAAG GCCTGTATAC CCTCAGACCA GTTTCTCAGA 961 ATTTTACAGC AGTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACATTCCCAC GGCCTACGTT 1141 GACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt