Construct: ORF TRCN0000480361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003561.1_s317c1
- Derived from:
- ccsbBroadEn_09187
- DNA Barcode:
- ACCCGATGCGGAAAGTACGGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYSTM1 (84418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84418 | CYSTM1 | cysteine rich transmembrane... | NM_032412.4 | 99.6% | 98.9% | 269G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 381
- ORF length:
- 291
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAACCAAGA GAACCCTCCA CCATATCCAG 121 GCCCTGGTCC AACGGCCCCA TACCCACCTT ATCCACCACA ACCAATGGGT CCAGGACCTA 181 TGGGGGGACC CTACCCACCT CCTCAAGGGT ACCCCTACCA AGGATACCCA CAGTACGGCT 241 GGCAGGGTGG ACCTCAGGAG CCTCCTAAAA CCACAGTGTA TGTGGTAGAA GACCAAAGAA 301 GAGATGAGCT AGGACCATCC ACCTGCCTCA CAGCCTGCTG GACGGCTCTC TGTTGCTCCT 361 GTCTCTGGGA CATGCTCACC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCC GATGCGGAAA 541 GTACGGACCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt