Construct: ORF TRCN0000480433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018417.1_s317c1
- Derived from:
- ccsbBroadEn_07863
- DNA Barcode:
- TGTTATACTGAGCGCATACGAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSTF2T (23283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23283 | CSTF2T | cleavage stimulation factor... | NM_015235.3 | 99.8% | 99.8% | 312T>C;1263T>C;1658A>G |
| 2 | mouse | 83410 | Cstf2t | cleavage stimulation factor... | NM_031249.2 | 83.5% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gagtttggcg gtgagagacc cggcaatgga tcgatcactg cgttccgtgt 121 tcgtggggaa cattccatat gaggcaactg aggagcagtt aaaggacatt ttctcggagg 181 ttggttctgt tgtcagtttc cggctggtat acgatagaga gacgggaaaa cccaagggct 241 atggcttctg cgaataccaa gaccaggaga ccgcgcttag tgccatgcgg aacctcaatg 301 ggcgggagtt cagtgggaga gcgcttcggg tggacaatgc tgccagtgaa aagaataagg 361 aggagttaaa gagcctcggg cctgcagcgc ccattattga ctcaccctat ggggatccca 421 tcgatccaga agatgcccct gaatcgatta ccagagcagt agccagtctc cccccggagc 481 agatgtttga gctgatgaag cagatgaagc tctgtgtcca aaacagccac caggaagctc 541 gaaacatgtt acttcaaaat ccacaactgg cttatgcact gttgcaggca caagtagtga 601 tgagaatcat ggatccagag attgctctga aaattctgca tcggaagata catgtcacac 661 cactgatccc aggcaaatct cagtctgtgt ctgtctctgg ccctggccct ggccctggcc 721 ctgggctctg cccaggacct aatgttctgc tgaaccagca gaatcctcca gctcctcagc 781 ctcagcattt ggctagaaga cctgtgaagg acattcctcc tctgatgcag actcctatcc 841 agggtggaat tccagctcca gggccaatac cagctgcagt tcccggagct ggtcctggtt 901 ccttaactcc tggaggagca atgcagcccc aacttggaat gccaggggtt ggcccagtgc 961 ctttagagcg gggacaagtg cagatgtcag atcctagagc tcctatacct cgcggacccg 1021 tgactcctgg tggtctgcct cctcgaggac tgttaggaga tgctccaaat gacccacgtg 1081 gagggacttt gctttcagtc actggagaag tggagcccag aggttatctg ggtccacccc 1141 atcagggtcc ccccatgcat catgcctctg gtcatgacac tcgtggccct tcctcacatg 1201 agatgagggg agggccatta ggagatccca gactgctaat tggagagccc agaggcccca 1261 tgatagatca aaggggtcta cctatggatg gtagaggtgg tagagattct cgagcgatgg 1321 agactcgcgc catggaaact gaggtcttag agacacgtgt aatggagagg agaggaatgg 1381 agacctgtgc gatggaaacc agagggatgg aagcaagggg catggatgca agaggattgg 1441 agatgagggg ccctgtcccc agttcaagag gccctatgac tggtggaatt cagggtcctg 1501 gtcccattaa tataggggca ggtggccctc cTCAGGGACC CAGACAGGTC CCAGGCATTT 1561 CAGGGGTGGG GAATCCTGGA GCTGGTATGC AGGGTACAGG CATACAAGGA ACAGGCATGC 1621 AGGGAGCAGG CATACAAGGA GGAGGGATGC AGGGGGCAGG CATACAAGGA GTCAGTATAC 1681 AAGGAGGAGG TATACAAGGA GGAGGTATAC AGGGGGCAAG CAGGCAAGGT GGAAGCCAGC 1741 CTAGCAGTTT TAGTCCTGGG CAGAGCCAGG TCACTCCACA GGATCAGGAG AAGGCAGCTT 1801 TGATCATGCA GGTTCTTCAA CTGACTGCAG ATCAGATTGC CATGCTGCCC CCTGAGCAAA 1861 GGCAGAGTAT CCTGATTTTA AAGGAACAAA TCCAGAAATC CACTGGAGCG TCTTGCCCAA 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAT GTTATACTGA GCGCATACGA ATGACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att