Construct: ORF TRCN0000480654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017179.1_s317c1
- Derived from:
- ccsbBroadEn_10662
- DNA Barcode:
- AGTTGAGCCGGCAGTCTGACCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC652276 (652276)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 652276 | LOC652276 | potassium channel tetrameri... | NR_015441.1 | 13.2% | 1_88del;449_2714del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 429
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgaTC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGCGGGGAAT CACTGCGAGC TCCTGCCGCT GGCCCGTGGC AGGCTCGGGG 121 CGGGGTTGGG GTGGCTTCTT GTGCCTCCCT TAAAGCGCGG GGCTCAGCGT CCTGGCCCAG 181 CGCCCCAGCA GCAGGTCCAA GTGGGTCCGG CTCTACAGCG GCGGCACCTA CTTCCTCACC 241 ACTGGGCAGA CGCCGCTGTG TCAGGACCCG AAATCCTTCC TGTACCTCTT GAGCCAGGCC 301 GACCCCGACC CGGACTCGGA CAAGACGGAG TTTTGTTCTT GTTGCCCAAG CTGGAGTACA 361 ATGGCACAAT CTTGGCTCAC CACAACCTCT GCCACCTGGG TTCAAGCGAG TCTCCTCCTT 421 CAGTCTCCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTTGA GCCGGCAGTC TGACCGGTAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt