Construct: ORF TRCN0000480715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017964.1_s317c1
- Derived from:
- ccsbBroadEn_10532
- DNA Barcode:
- GCTGTTACCTAGGTTGTTCCGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TENM3-AS1 (90768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480715
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90768 | TENM3-AS1 | TENM3 antisense RNA 1 | NR_027107.2 | 8.3% | 1_1036del;1217T>G;1481_5306del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgacctgagc cgaaccgctc tctggctggg tggtcggcgg gatccttcag 121 gctcctttcG GGTCCGGGAC GGGGCGGCGG CAGAGGTGGG GCGTGCGTGC TTGGGGCTGC 181 CGAGTGAGTG CGGCTCCCTG GTGCGCGCCC GAGCATCTCC CCGCCCTCTC CTGGGACCAG 241 CAGTCGGCCC GTGGAGGGCT AGGAGCCCGC GACCAGGCCT GCCCCGGCCG TCGCCAACCT 301 GTCCTTGGAA GCGTGGCGGA GACTGGTGCG CTCTCAGCCT CGTCGCGCCA GCCGCCTCGC 361 GCCACCCTCC ACTTTCAGCT TCAGAGCAGA GGATTCCGCC GACCTCCACC TGTACCTGTA 421 CTCGGCCGTT AGGAAATCCA CTTGCATTTT TAACTACCGT GCAGAACCAG AAACAGATCG 481 AGCACTGGGA GAACAAGGAC GACGCCTCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCTGT 661 TACCTAGGTT GTTCCGCCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 721 tgaaagatt