Construct: ORF TRCN0000480800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014654.1_s317c1
- Derived from:
- ccsbBroadEn_10471
- DNA Barcode:
- TTATATTTTGTGGTGTCCGTAACC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCAB7 (84455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | NM_032437.4 | 99.7% | 99.3% | (many diffs) |
| 2 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XM_006710977.1 | 99.7% | 99.3% | (many diffs) |
| 3 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XM_006710976.3 | 97.7% | 97.3% | (many diffs) |
| 4 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XM_005271274.4 | 96% | 95.7% | (many diffs) |
| 5 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XM_011542301.2 | 94.1% | 93.7% | (many diffs) |
| 6 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XR_001737487.2 | 79% | (many diffs) | |
| 7 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XR_001737486.2 | 68.7% | (many diffs) | |
| 8 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XR_946779.3 | 59.4% | (many diffs) | |
| 9 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XM_017002548.1 | 58.5% | 54.9% | (many diffs) |
| 10 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XR_001737488.1 | 55.1% | (many diffs) | |
| 11 | human | 84455 | EFCAB7 | EF-hand calcium binding dom... | XR_002957809.1 | 49.2% | (many diffs) | |
| 12 | mouse | 230500 | Efcab7 | EF-hand calcium binding dom... | NM_145549.1 | 85.6% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1953
- ORF length:
- 1887
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gatcagtcca cgaagcgatg caactttctc cagtcagaaa tcaacacctt 121 cagagagtcc tcgaacaaag aaatttccac taactgaaga ggaaatattt tatatgaatt 181 gtagagctgc ctacttaact gtcttcaaaa gcagcttgga aaacattatt tctaaagatc 241 aactttactt agctcttcag catgcaggaa gaaatccatc ccaaaagacc attaataagt 301 attggactcc tcaaactgcc aaactgaatt ttgatgattt ttgtataatt ttaaggaagg 361 aaaaacctac ttcaaaagca gaactactaa aatcttttaa gcaattagat gtaaatgatg 421 atggctgtat tttacacact gacctttata aatttctaac aaagagaggt gagaagatga 481 ctcgagaaga agtaaatgcc ataataaatt tggctgatgt aaatgctgat ggcaaatttg 541 actacatcaa gttttgtaaa ttatatatga caaccaacga gcaatgtctc aagactacac 601 tagaaaaact agaggttgac agtaaattga tgcgtcacca gtttggaaac cacatcgaag 661 ggtcccctga aagggaccca tcaccagtac caaaaccatc acctaaaatc acaagaaaaa 721 ctgatccaga aacattctta aataaaggtg acaccaggag ttctttactg tcagcaacca 781 ggaagttcaa aacatctgtt tccttcatag ttaccatggg ggctaatggt aaccgaaact 841 caaagttaac ggagccaaat ttaataaagg actggcaaca catgcaatca aaaggttgct 901 tcttcttaga agaagatggt gaaatcatta gtcatcagta caggatgcaa atagctcaga 961 ggtccatggt ttatctaaca attaagccat taaacctgag tcaagttgaa ggaaaaccat 1021 ccccttggtt atccgttgat actgccttgt atattctcaa ggaaaatgag agtcaagcaa 1081 atctacagct tgtgtgtttt accgaactac gaaatagaga agtgtttgga tggactggtg 1141 aactaggacc tggaatttac tggttaattc cttccacaac tggctgtaag ctgaggaaaa 1201 aaataaaacc agtaacagat gaagcccaac ttgtatatag agatgaaaca ggggaattat 1261 tccttacaaa ggaatttaag tctactttat cagatatatt tgaagtaatt gatttagatg 1321 gaaatggtct tcttagcctt gaagaatata atttttttga attgagaaca agtggtgaga 1381 aatgtgatga agatgcttgg gctgtctgca gagagaattt tgatacaaag aggaatgaac 1441 taacaagaca aggatttatg gatttgaatc taatggaagc taatgatcga gaaggagatc 1501 cttgtgacct ttgggtaact ctacactcta tgggctaCAA TAAAGCTCTG GAGTTGACAG 1561 AGGCATGTCC ATTTGTCATT GATATCTATG CAGAAAAATG CAAGCCAAAA ATTAAAGCTG 1621 TCCATATGGA GGCATGTAGT GGACAACTTG AGAAGGCCAT TTGTAAATCT GTTCTTAGCA 1681 ACGGTGATGC CAAAGTAATG GATGGCTATG AAAATATAAT CGTGCATACT TACAGTTGTG 1741 ACACCTGGAT AACGTCAGTT ATTGAAAACA AGTCAGATGA GAAAGTGATT ATTCACATCA 1801 GCAATGAGCT AAGTAAAAAC TGCATAAACA ACAGAGGACT CAACATATTT GCAGTAGAAG 1861 TGGGACCCAA ATCTACAATG GTTTGTCAAC ATGTAATGCC TTTGAATGAA CGACAAGAAT 1921 GGATATATTA TTGTATATAT TCTCTTATTC TTACCCAACT TTCTTGTACA AAGTGGTTGA 1981 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 2041 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTA 2101 TATTTTGTGG TGTCCGTAAC CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 2161 tgtgaaagat t