Construct: ORF TRCN0000480824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002970.1_s317c1
- Derived from:
- ccsbBroadEn_05999
- DNA Barcode:
- TTCCCCGTTCCTAGAAGGGGCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM5 (1133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1133 | CHRM5 | cholinergic receptor muscar... | NM_001320917.2 | 99.8% | 99.8% | 528G>T;635T>C |
| 2 | human | 1133 | CHRM5 | cholinergic receptor muscar... | NM_012125.4 | 99.8% | 99.8% | 528G>T;635T>C |
| 3 | mouse | 213788 | Chrm5 | cholinergic receptor, musca... | NM_205783.2 | 88.1% | 88.5% | (many diffs) |
| 4 | mouse | 213788 | Chrm5 | cholinergic receptor, musca... | XM_006499099.3 | 88.1% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggggattct taccacaatg caaccaccgt caatggcacc ccagtaaatc 121 accagccttt ggaacgccac aggttgtggg aagtcatcac cattgcagct gtgactgctg 181 tggtaagcct gatcaccatt gtgggcaatg tcttggtcat gatctccttc aaagtcaaca 241 gccagctcaa gacagttaac aactattacc tgctcagctt agcctgtgca gatctcatca 301 ttggaatctt ctccatgaac ctctacacca cctacatcct catgggacgc tgggctctcg 361 ggagtctggc ttgtgacctt tggcttgcac tggactacgt ggccagcaac gcttctgtca 421 tgaaccttct ggtgatcagt tttgaccgtt acttttccat cacaagaccc ttgacatatc 481 gggccaagcg tactccgaaa agggctggca tcatgattgg cttggcctgg ctgatctcct 541 tcatcctctg ggccccagca atcctctgct ggcagtactt ggttgggaag cgtacagttc 601 cactggatga gtgccagatc cagtttctct ctgagcccac catcactttt ggcactgcca 661 ttgctgcctt ctacatccct gtttctgtca tgaccatccc ctactgtcga atctaccggg 721 aaacagagaa gcgaaccaag gacctggctg acctccaggg ttctgactct gtgaccaaag 781 ctgagaagag aaagccagct catagggctc tgttcagatc ctgcttgcgc tgtcctcgac 841 ccaccctggc ccagcgggaa aggaaccagg cctcctggtc atcctcccgc aggagcacct 901 ccaccactgg gaagccatcc caagccactg gcccaagcgc caattgggcc aaagctgagc 961 agctcaccac ctgtagcagc tacccttcct cagaggatga ggacaagccc gccactgacc 1021 ctgtcctcca agtggtctac aagagtcagg gtaaggaaag cccaggggaa gaattcagtg 1081 ctgaagagac tgaggaaact tttgtgaaag ctgaaactga aaaaagtgac tatgacaccc 1141 caaactacct tctgtctcca gcagctgcTC ATAGACCCAA GAGTCAGAAA TGTGTGGCCT 1201 ATAAGTTCCG ATTGGTGGTA AAAGCTGACG GGAACCAGGA GACCAACAAT GGCTGTCACA 1261 AGGTGAAAAT CATGCCCTGC CCCTTCCCAG TGGCCAAGGA ACCTTCAACG AAAGGCCTCA 1321 ATCCCAACCC CAGCCATCAA ATGACCAAAC GAAAGAGAGT GGTCCTAGTC AAAGAGAGGA 1381 AAGCAGCCCA GACACTGAGT GCCATTCTCC TGGCCTTCAT CATCACATGG ACCCCGTATA 1441 ACATCATGGT CCTGGTTTCT ACCTTCTGTG ACAAGTGTGT CCCAGTCACC CTGTGGCACT 1501 TGGGCTATTG GTTGTGCTAT GTCAATAGCA CTGTCAACCC CATCTGCTAT GCCCTCTGCA 1561 ACAGAACCTT CAGGAAGACC TTTAAGATGC TGCTTCTCTG CCGATGGAAA AAGAAAAAAG 1621 TGGAAGAGAA GTTGTACTGG CAGGGGAACA GCAAGCTACC CTGCCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGATTC CCCGTTCCTA GAAGGGGCAA AACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t