Construct: ORF TRCN0000480828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001356.1_s317c1
- Derived from:
- ccsbBroadEn_06799
- DNA Barcode:
- AATATATCATAAAGAAGTAACAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMB4 (5692)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5692 | PSMB4 | proteasome 20S subunit beta 4 | NM_002796.3 | 99.8% | 100% | 75G>A |
| 2 | mouse | 19172 | Psmb4 | proteasome (prosome, macrop... | NM_008945.3 | 87.7% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agcgtttttg gggtcgcggt ccggactttg ggcggggggt ccggccccag 121 gacagtttta ccgcattcca tccactcccg attccttcat ggatccggcg tctgcacttt 181 acagaggtcc aatcacgcgg acccagaacc ccatggtgac cgggacctca gtcctcggcg 241 ttaagttcga gggcggagtg gtgattgccg cagacatgct gggatcctac ggctccttgg 301 ctcgtttccg caacatctct cgcattatgc gagtcaacaa cagtaccatg ctgggtgcct 361 ctggcgacta cgctgatttc cagtatttga agcaagttct cggccagatg gtgattgatg 421 aggagcttct gggagatGGA CACAGCTATA GTCCTAGAGC TATTCATTCA TGGCTGACCA 481 GGGCCATGTA CAGCCGGCGC TCGAAGATGA ACCCTTTGTG GAACACCATG GTCATCGGAG 541 GCTATGCTGA TGGAGAGAGC TTCCTCGGTT ATGTGGACAT GCTTGGTGTA GCCTATGAAG 601 CCCCTTCGCT GGCCACTGGT TATGGTGCAT ACTTGGCTCA GCCTCTGCTG CGAGAAGTTC 661 TGGAGAAGCA GCCAGTGCTA AGCCAGACCG AGGCCCGCGA CTTAGTAGAA CGCTGCATGC 721 GAGTGCTGTA CTACCGAGAT GCCCGTTCTT ACAACCGGTT TCAAATCGCC ACTGTCACCG 781 AAAAAGGTGT TGAAATAGAG GGACCATTGT CTACAGAGAC CAACTGGGAT ATTGCCCACA 841 TGATCAGTGG CTTTGAATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATATAT CATAAAGAAG 1021 TAACAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt