Construct: ORF TRCN0000480859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005503.1_s317c1
- Derived from:
- ccsbBroadEn_01054
- DNA Barcode:
- ATTGGCCCCCGGCTTCCAAACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GADD45B (4616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480859
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4616 | GADD45B | growth arrest and DNA damag... | NM_015675.4 | 100% | 100% | |
| 2 | human | 4616 | GADD45B | growth arrest and DNA damag... | XM_017026822.1 | 79.2% | 68.9% | (many diffs) |
| 3 | mouse | 17873 | Gadd45b | growth arrest and DNA-damag... | NM_008655.1 | 86.2% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 549
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacgctggaa gagctcgtgg cgtgcgacaa cgcggcgcag aagatgcaga 121 cggtgaccgc cgcggtggag gagcttttgg tggccgcTCA GCGCCAGGAT CGCCTCACAG 181 TGGGGGTGTA CGAGTCGGCC AAGTTGATGA ATGTGGACCC AGACAGCGTG GTCCTCTGCC 241 TCTTGGCCAT TGACGAGGAG GAGGAGGATG ACATCGCCCT GCAAATCCAC TTCACGCTCA 301 TCCAGTCCTT CTGCTGTGAC AACGACATCA ACATCGTGCG GGTGTCGGGC ATGCAGCGCC 361 TGGCGCAGCT CCTGGGAGAG CCGGCCGAGA CCCAGGGCAC CACCGAGGCC CGAGACCTGC 421 ATTGTCTCCT GGTCACGAAC CCTCACACGG ACGCCTGGAA GAGCCACGGC TTGGTGGAGG 481 TGGCCAGCTA CTGCGAAGAA AGCCGGGGCA ACAACCAGTG GGTCCCCTAC ATCTCTCTTC 541 AGGAACGCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 601 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 661 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATTGGC CCCCGGCTTC CAAACACAAC 721 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt