Construct: ORF TRCN0000480874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016470.1_s317c1
- Derived from:
- ccsbBroadEn_06075
- DNA Barcode:
- TCCTCTTACGCCACAGACACAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2E1 (1571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480874
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1571 | CYP2E1 | cytochrome P450 family 2 su... | NM_000773.4 | 99.8% | 99.5% | 361A>G;1334T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgccctcgga gtcaccgtgg ccctgctggt gtgggcggcc ttcctcctgc 121 tggtgtccat gtggaggcag gtgcacagca gctggaatct gcccccaggc cctttcccgc 181 ttcccatcat cgggaacctc ttccagttgg aattgaagaa tattcccaag tccttcaccc 241 ggttggccca gcgcttcggg ccggtgttca cgctgtacgt gggctcgcag cgcatggtgg 301 tgatgcacgg ctacaaggcg gtgaaggaag cgctgctgga ctacaaggac gagttctcgg 361 gcagaggcga cctccccgcg ttccatgcgc acagggacag gggaatcatt tttaataatg 421 gacctgcctg gaaggacatc cggcggtttt ccctgaccac cctccggaac tatgggatgg 481 ggaaacaggg caatgagagc cggatccaga gggaggccca cttcctgctg gaagcactca 541 ggaagaccca aggccagcct ttcgacccca ccttcctcat cggctgcgcg ccctgcaacg 601 tcatagccga catcctcttc cgcaagcatt ttgactacaa tgatgagaag tttctaaggc 661 tgatgtattt gtttaatgag aacttccacc tactcagcac tccctggctc cagctttaca 721 ataattttcc cagctttcta cactacttgc ctggaagcca cagaaaagtc ataaaaaatg 781 tggctgaagt aaaagagtat gtgtctgaaa gggtgaagga gcaccatcaa tctctggacc 841 ccaactgtcc ccgggacctc accgactgcc tgctcgtgga aatggagaag gaaaagcaca 901 gtgcagagcg cttgtacaca atggacggta tcaccgtgac tgtggccgac ctgttctttg 961 cggggacaga gaccaccagc acaactctga gatatgggct cctgattctc atgaaatacc 1021 ctgagatcga agagaagctc catgaagaaa ttgacagggt gattgggcca agccgaatcc 1081 ctgccatcaa ggataggCAA GAGATGCCCT ACATGGATGC TGTGGTGCAT GAGATTCAGC 1141 GGTTCATCAC CCTCGTGCCC TCCAACCTGC CCCATGAAGC AACCCGAGAC ACCATTTTCA 1201 GAGGATACCT CATCCCCAAG GGCACAGTCG TAGTGCCAAC TCTGGACTCT GTTTTGTATG 1261 ACAACCAAGA ATTTCCTGAT CCAGAAAAGT TTAAGCCAGA ACACTTCCTG AATGAAAATG 1321 GAAAGTTCAA GTACAGTGAC TATTTCAAGC CATTTTCCAC AGGAAAACGA GTGTGTGCTG 1381 GAGAAGGCCT GGCTCGCACG GAGTTGTTTC TTTTGTTGTG TGCCATTTTG CAGCATTTTA 1441 ATTTGAAGCC TCTCGTTGAC CCAAAGGATA TCGACCTCAG CCCTATACAT ATTGGGTTTG 1501 GCTGTATCCC ACCACGTTAC AAACTCTGTG TCATTCCCCG CTCATGCCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TCCTCTTACG CCACAGACAC AGACACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt