Construct: ORF TRCN0000480905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006222.1_s317c1
- Derived from:
- ccsbBroadEn_03534
- DNA Barcode:
- TAGGGCGTAGACGACCCTTAATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THAP1 (55145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55145 | THAP1 | THAP domain containing 1 | NM_018105.3 | 100% | 100% | |
| 2 | human | 55145 | THAP1 | THAP domain containing 1 | NM_199003.1 | 24.8% | 12.1% | 71_72ins196;159_160ins284 |
| 3 | mouse | 73754 | Thap1 | THAP domain containing, apo... | NM_199042.2 | 83.8% | 92.4% | (many diffs) |
| 4 | mouse | 73754 | Thap1 | THAP domain containing, apo... | XM_006509202.3 | 73.2% | 81.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcagtcctgc tccgcctacg gctgcaagaa ccgctacgac aaggacaagc 121 ccgtttcttt ccacaagttt cctcttactc gacccagtct ttgtaaagaa tGGGAGGCAG 181 CTGTCAGAAG AAAAAACTTT AAACCCACCA AGTATAGCAG TATTTGTTCA GAGCACTTTA 241 CTCCAGACTG CTTTAAGAGA GAGTGCAACA ACAAGTTACT GAAAGAGAAT GCTGTGCCCA 301 CAATATTTCT TTGTACTGAG CCACATGACA AGAAAGAAGA TCTTCTGGAG CCACAGGAAC 361 AGCTTCCCCC ACCTCCTTTA CCGCCTCCTG TTTCCCAGGT TGATGCTGCT ATTGGATTAC 421 TAATGCCGCC TCTTCAGACC CCTGTTAATC TCTCAGTTTT CTGTGACCAC AACTATACTG 481 TGGAGGATAC AATGCACCAG CGGAAAAGGA TTCATCAGCT AGAACAGCAA GTTGAAAAAC 541 TCAGAAAGAA GCTCAAGACC GCACAGCAGC GATGCAGAAG GCAAGAACGG CAGCTTGAAA 601 AATTAAAGGA GGTTGTTCAC TTCCAGAAAG AGAAAGACGA CGTATCAGAA AGAGGTTATG 661 TGATTCTACC AAATGACTAC TTTGAAATAG TTGAAGTACC AGCATACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TAGGGCGTAG ACGACCCTTA ATAAACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt