Construct: ORF TRCN0000481017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005185.1_s317c1
- Derived from:
- ccsbBroadEn_07100
- DNA Barcode:
- TCCCGTGCCGAGTATGACATACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPC5 (7224)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7224 | TRPC5 | transient receptor potentia... | NM_012471.2 | 99.9% | 100% | 1998C>T |
| 2 | human | 7224 | TRPC5 | transient receptor potentia... | XM_017029774.1 | 99.9% | 100% | 1998C>T |
| 3 | mouse | 22067 | Trpc5 | transient receptor potentia... | NM_009428.3 | 90.1% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2988
- ORF length:
- 2919
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccaactg tactacaaaa aggtcaacta ctcaccgtac agagaccgca 121 tccccctgca aattgtgagg gctgagacag agctctctgc agaggagaag gccttcctca 181 atgctgtgga gaagggggac tatgccactg tgaagcaggc ccttcaggag gctgagatct 241 actataatgt taacatcaac tgcatggacc ccttgggccg gagtgccctg ctcattgcca 301 ttgagaacga gaacctggag atcatggagc tactgctgaa ccacagcgtg tatgtgggtg 361 atgcattgct ctatgccata cgcaaggaag tggtgggcgc tgtggagctt ctgctcagct 421 acaggcggcc cagcggagag aagcaggtcc ccactctgat gatggacacg cagttctctg 481 aattcacacc ggacatcact cccatcatgc tggctgccca caccaacaac tacgaaatca 541 tcaaattgct tgtccaaaaa cgggtcacta tcccacggcc ccaccagatc cgctgcaact 601 gtgtggagtg tgtgtctagt tcagaggtag acagcctgcg ccactctcgc tcccgactga 661 acatctataa ggctctggca agcccctcac tcattgcctt atcaagtgag gaccccatcc 721 taactgcctt ccgtctgggc tgggagctca aggagctcag caaggtggag aatgagttca 781 aggccgagta tgaggagctc tctcagcagt gcaagctctt tgccaaagac ctgctggacc 841 aagctcggag ctccagggaa ctggagatca tcctcaacca tcgagatgac cacagtgaag 901 agcttgaccc tcagaagtac catgacctgg ccaagttgaa ggtggcaatc aaataccacc 961 agaaagagtt tgttgctcag cccaactgcc aacagttgct tgccaccctg tggtatgatg 1021 gcttccctgg atggcggcgg aaacactggg tagtcaagct tctaacctgc atgaccattg 1081 ggttcctgtt tcccatgctg tctatagcct acctgatctc acccaggagc aaccttgggc 1141 tgttcatcaa gaaacccttt atcaagttta tctgccacac agcatcctat ttgaccttcc 1201 tctttatgct tctcctggct tctcagcaca ttgtcaggac agaccttcat gtacaggggc 1261 ctcccccaac tgtcgtggaa tggatgatat tgccttgggt tctaggtttc atttggggtg 1321 agattaagga aatgtgggat ggtggattta ctgaatacat ccatgactgg tggaacctga 1381 tggattttgc aatgaactcc ctctacctgg caactatttc cctgaagatt gtggcctatg 1441 tcaagtataa tggttctcgt ccaagggagg aatgggaaat gtggcacccg actctgattg 1501 cggaagcact cttcgcaata tccaacattt taagttcgtt gcgtctcata tccctgttca 1561 cagccaactc ccacttagga cctctgcaga tctctttggg acgcatgctg cttgatatcc 1621 tcaaattcct ctttatctac tgcctggtac tactagcttt tgccaatgga ctgaaccagc 1681 tttacttcta ttatgaaacc agagctatcg atgagcctaa caactgcaag gggatccgat 1741 gtgagaaaca gaacaatgcc ttctccacgc tctttgagac tcttcagtca ctcttctggt 1801 ctgtatttgg ccttttaaat ctatatgtca ccaatgtgaa agccagacac gaattcaccg 1861 agtttgtagg agctaccatg tttggaacat acaatgtcat ctccctggta gtgctgctga 1921 acatgctgat tgctatgatg aacaactcct atcagcttat tgccgatcat gctgatatcg 1981 agtggaagtt tgcaaggacg aagctctgga tgagttactt tgatgaaggt ggcaccttgc 2041 cacctccttt caacatcatc cccagtccca agtcatttct ataccttggt aactggttca 2101 acaacacctt ctgccccaaa agagaccctg acggtagacg gagaaggcgc aacttgagaa 2161 gtttcacaga acgcaatgct gacagcctga tacaaaatca acattatcag gaagttatca 2221 ggaatttagt caaaagatat gtggctgcta tgataagaaa ttccaaaaca catgagggac 2281 ttacagaaga aaattttaag gaattaaagc aagacatctc cagctttcgg tatgaagtgc 2341 ttgacctctt gggaaataga aaacatccaa ggagcttttc cactagcagc actgaactgt 2401 ctcagagaga cgataataat gatggcagtg gtggggctcg ggccaaatcc aagagtgtct 2461 cttttaattt aggctgcaag aaaaagactt gccatgggcc acctctcatc agaaccatgc 2521 caaggtccag tggtgcccaa ggaaagtcaa aagctgagtc atcaagcaaa cgctccttca 2581 tgggtccTTC TCTCAAGAAA CTGGGTCTCC TATTCTCCAA ATTTAATGGT CATATGTCTG 2641 AACCCAGTTC AGAGCCAATG TACACAATTT CTGATGGAAT TGTTCAGCAG CACTGTATGT 2701 GGCAGGACAT CAGATATTCT CAGATGGAGA AAGGGAAAGC AGAGGCCTGT TCTCAAAGTG 2761 AAATTAACCT CAGTGAGGTA GAATTAGGTG AAGTCCAGGG CGCTGCTCAG AGCAGTGAAT 2821 GCCCTCTAGC CTGTTCCAGC TCTCTTCACT GTGCATCCAG CATCTGCTCC TCAAATTCTA 2881 AACTTTTAGA CTCCTCAGAG GATGTATTTG AAACTTGGGG AGAGGCTTGT GACTTGCTCA 2941 TGCACAAATG GGGTGATGGA CAGGAAGAAC AAGTTACAAC TCGCCTCTTG CCAACTTTCT 3001 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3061 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3121 GTGGAAAGGA CGATCCCGTG CCGAGTATGA CATACCGACG CGTTAAGTCg acaatcaacc 3181 tctggattac aaaatttgtg aaagatt