Construct: ORF TRCN0000481032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016546.1_s317c1
- Derived from:
- ccsbBroadEn_05658
- DNA Barcode:
- AGATGGCGCCCTGCCCACAACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERVFRD-1 (405754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 405754 | ERVFRD-1 | endogenous retrovirus group... | NM_207582.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cctgctcctg ctggttctca ttctcacgcc ttcactagca gcctaccgcc 121 atcctgattt cccgttattg gaaaaagctc agcaactgct ccaaagtaca ggatcccctt 181 actccaccaa ttgctggtta tgtactagct cttccactga aacaccaggg acagcttatc 241 cagcctcgcc cagagaatgg acaagcatag aggcggaatt acatatttcc tatcgatggg 301 accctaatct gaaaggactg atgaggcctg caaatagtct tctttcaaca gtaaagcaag 361 atttccctga tatccgccag aaacctccca ttttcggacc catctttact aatatcaacc 421 taatgggaat agcccctatt tgtgttatgg ccaaaaggaa aaatggaaca aatgtaggca 481 ctcttccaag tacagtctgt aatgttactt tcactgtaga ttctaaccaa cagacttacc 541 aaacatacac ccacaaccaa ttccgccatc aaccaagatt ccccaaacct ccaaatatta 601 cttttcctca gggaactttg ctagataaat ccagccggtt ttgccaggga cgcccaagct 661 catgcagtac tcgaaacttc tggttccggc ctgctgatta taaccaatgt ctgcaaattt 721 ccaacctcag ctctacagcg gaatgggttc tattggacca aactcgaaat tctctttttt 781 gggaaaataa aaccaaggga gctaaccaga gccaaacacc ctgcgtccaa gtcttagcag 841 gcatgactat agccaccagc tacctgggca tatcagcagt ctcagaattt tttggaacct 901 ccctcacccc cttatttcat ttccatatct ctacatgcct taaaactcaa ggagcctttt 961 atatttgtgg ccagtcgatt caccaatgcc tccccagtaa ctggactgga acttgtacca 1021 taggctatgt aaccccagac atcttcatag cccctggcaa tctctctctt ccaataccaa 1081 tctatgggaa ttccccgttg cccagggtga ggagggcaat ccatttcatt ccccttctcg 1141 cgggactcgg cattctagct ggtacgggaa ccggaattgc tggaatcaca aaagcttccc 1201 tcacctatag ccagctctca aaggaaatag ccaacaacat tgacaccatg gctaaagcct 1261 taacgaccat gcaagaacaa atcgactctt tagcagccGT AGTCCTTCAA AATCGTCGAG 1321 GACTAGACAT GTTAACGGCA GCACAGGGAG GAATTTGTTT GGCCTTAGAT GAAAAATGTT 1381 GCTTTTGGGT AAATCAATCA GGAAAAGTAC AAGACAACAT CAGACAACTC CTAAATCAAG 1441 CCTCCAGTTT ACGGGAACGA GCCACTCAGG GTTGGTTAAA TTGGGAAGGA ACTTGGAAAT 1501 GGTTCTCTTG GGTTCTTCCC CTTACAGGCC CACTTGTTAG TCTCCTACTT TTGCTCCTTT 1561 TTGGTCCATG TCTCCTAAAT CTAATAACCC AATTTGTCTC CTCTCGCCTT CAGGCCATAA 1621 AGCTCCAGAC GAATCTCAGT GCAGGACGCC ATCCTCGCAA TATTCAAGAG TCACCCTTCT 1681 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGAAGATG GCGCCCTGCC CACAACTGTA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt