Construct: ORF TRCN0000481114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007571.1_s317c1
- Derived from:
- ccsbBroadEn_12964
- DNA Barcode:
- TCCAAACGCTTTTCTGCCTGTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHURC1 (91612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481114
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91612 | CHURC1 | churchill domain containing 1 | NM_001204063.1 | 80.5% | 80.5% | 1_81del |
| 2 | human | 91612 | CHURC1 | churchill domain containing 1 | NM_145165.3 | 80% | 79.2% | 1_81del;257_259delCAG |
| 3 | human | 91612 | CHURC1 | churchill domain containing 1 | NM_001204064.1 | 56.1% | 43% | 1_81del;256_257ins71;315_316ins31 |
| 4 | human | 100529261 | CHURC1-FNTB | CHURC1-FNTB readthrough | NM_001202559.1 | 20.5% | 16.6% | (many diffs) |
| 5 | mouse | 211151 | Churc1 | churchill domain containing 1 | NM_206534.1 | 90.7% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 402
- ORF length:
- 336
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg taCAAAAAAG 61 TTGGCATGTG TGGCGACTGT GTGGAGAAGG AATATCCCAA CCGGGGTAAT ACCTGCCTGG 121 AGAATGGATC TTTCTTACTG AACTTTACAG GCTGTGCAGT GTGCAGTAAG CGGGATTTTA 181 TGCTGATCAC AAACAAATCC TTGAAAGAAG AAGATGGAGA AGAAATAGTT ACCTATGATC 241 ATTTGTGTAA GAATTGTCAT CATGTAATAG CCAGACATGA GTATACATTC AGTATCATGG 301 ATGAATTTCA GGAGTATACC ATGCTGTGTC TGTTATGCGG CAAAGCCGAA GATACTATCA 361 GTATTCTCCC TGATGACCCC CGACAAATGA CTCTCTTATT CTACCCAACT TTCTTGTACA 421 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 481 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 541 AGGACGATCC AAACGCTTTT CTGCCTGTAT AACGCGTTAA GTCgacaatc aacctctgga 601 ttacaaaatt tgtgaaagat t