Construct: ORF TRCN0000481163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012426.1_s317c1
- Derived from:
- ccsbBroadEn_14455
- DNA Barcode:
- GCATAGAAGATCACATGCCTCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCERG1L (256536)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256536 | TCERG1L | transcription elongation re... | NM_174937.4 | 92.7% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1701
- ORF length:
- 1635
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gccgggctcg gccgggctgc tccggctcag cgcgggggtc gtggttcccc 121 cggtgctgct cgcctcggcc ccgccgcccg cggccccgct gctccccggt ctccccggct 181 ggccggcccc gagcgagccg gtgctcccgc tgctgccgct gccctctgcg ccagactccg 241 ccgccgccgc cgccgcgcac cccttccccg cgctccacgg gcagtggctg tttggtggcc 301 attctccgtc cctaggactg cccccctctt ccacagtgga gctggtgccc gtcttcccac 361 atctctgccc ttctgctctt gcaaccccta ttgggaaaag ttggatagac aaaaggattc 421 ctaactgtaa gatctttttt aataattcct ttgctctgga ctcaacgtgg atacatcctg 481 aggagtcaag gtttttccat gggcatgaaa agcctcgttt gctggcaaat caagtagctg 541 tgtctctgtc caggccggct cctgcctcca ggccgctccc cacggtggtg ttagcacctc 601 agcccatccc aggtggctgc cataacagcc ttaaggtgac cagcagcccc gccattgcca 661 tcgccaccgc cgccgccgct gccatggtct ccgtggaccc tgagaacctc cggggcccgt 721 ccccctccag cgtgcagccg cgccacttcc tgaccttggc acccatcaaa atacccctcc 781 ggacgtcccc cgtctcagat acaaggacag agcggggccg agtggcccgc cctcctgccc 841 tgatgctgcg ggcccagaag agccgggatg gagacaaaga agacaaggag cctccaccga 901 tgctgggggg aggagaggac agcacagcca gaggcaacag gccagtggcc tccacccagg 961 tgcccggatc cccctggtgt gtggtctgga cgggcnatga ccgagttttc ttcttcaacc 1021 caacgatgca cctgtctgtc tgggagaagc ccatggacct gaaggaccgc ggagacctca 1081 acaggatcat tgaggacccg ccccacaaac gcaagctgga ggcaccagca actgacaaca 1141 gcgatgggtc cagttctgaa gacaacaggg aagaccaaga tgtgaaaacc aagaggaacc 1201 ggaccgaagg ctgcgggagt cccaagccag aggaggcaaa gagagaggac aaaggcacaa 1261 ggacgccgcc cccgcagatc ctcctgccTC TGGAGGAGCG TGTGACCCAC TTCCGAGACA 1321 TGCTGCTGGA GAGAGGGGTA TCAGCATTTT CTACCTGGGA GAAAGAATTA CACAAAATCG 1381 TGTTTGACCC ACGCTATCTC CTGCTCAACT CTGAGGAACG AAAGCAGATA TTTGAACAGT 1441 TTGTCAAGAC AAGAATAAAA GAAGAATACA AGGAAAAGAA AAGTAAATTG CTGCTAGCCA 1501 AAGAAGAATT CAAGAAACTT CTAGAGAAAT CTAAAGTGTC TCCCAGGACC ACGTTTAAGG 1561 AGTTTGCAGA GAAATACGGC CGGGATCAGA GGTTCCGACT TGTTCAAAAA AGAAAGGACC 1621 AGGAGCATTT TTTCAACAAA TTCATACTTA TTCTTAAGAA ACGGGACAAG GAAAACAGAC 1681 TAAGGCTGCG GAAAATGAGA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1741 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1801 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAT AGAAGATCAC 1861 ATGCCTCTCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt