Construct: ORF TRCN0000481170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008830.1_s317c1
- Derived from:
- ccsbBroadEn_12997
- DNA Barcode:
- CCACTGCGTGCACGGGTACCATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFEM1P (93556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93556 | EGFEM1P | EGF like and EMI domain con... | NR_021485.2 | 14.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 654
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgaacta agatggtatc acattacagt ctgtttagat cacatctttg 121 gacacaattg cagcttgagc tgcaaggact gcatgaatgg aggaaagtgc caggaaggaa 181 agagtgaatg ctcctgccca gcaggttgcc gtgtcattct ttgtaatgaa aactgtctcg 241 agggtgccTA CGGAGCTGGC TGCACTTCAG AATGTCAGTG TGTGGAGGAG AATACCCTGG 301 AATGCAGTGC CAAAAATGGT AGTTGCACCT GCAAATCTGG TTACCAAGGC AACAGATGCC 361 AGAAAGATGG CCTCTGGGGA CCAGAGGGCT GGTTCTCCTC TGCACCCTGT GAAAATGGAG 421 GTCAGTGCAA CAAAAAAACT GGAAATTGTG ACTGCACTCC TGATTACACA AGAAAATCCT 481 GTACCATATT GAGATGCATT TCCCTTACCA ATCTGGCTCT CAGCAGGCGG TCCAGCCCGA 541 TGAAGTATCA GCAAAATGTC AGCTCCCACA GAGAAGTGCG ACAGAGGCAA CAGTGCAGCA 601 GTGACAGGCC TTTCAAAAAG CTATTGTGCA AATTCAGTTT TAAAATAGGG ATGTTGCCAA 661 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAC CACTGCGTGC ACGGGTACCA TGCACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att