Construct: ORF TRCN0000481173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007868.1_s317c1
- Derived from:
- ccsbBroadEn_09009
- DNA Barcode:
- CTCTACCGGTGATGCGTCAGTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPSB1 (80176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80176 | SPSB1 | splA/ryanodine receptor dom... | NM_025106.4 | 99.7% | 99.2% | 4G>T;14T>G |
| 2 | mouse | 74646 | Spsb1 | splA/ryanodine receptor dom... | NM_029035.2 | 88.4% | 97.8% | (many diffs) |
| 3 | mouse | 74646 | Spsb1 | splA/ryanodine receptor dom... | XM_006539234.2 | 88.4% | 97.8% | (many diffs) |
| 4 | mouse | 74646 | Spsb1 | splA/ryanodine receptor dom... | XM_011250344.2 | 88.4% | 97.8% | (many diffs) |
| 5 | mouse | 74646 | Spsb1 | splA/ryanodine receptor dom... | XM_011250345.2 | 88.4% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tcagaagggc actggaggga tcaagactgt ggacatgagg gaccccacgt 121 acaggcccct gaagcaggag ctccagggtc tggattactg caagcccacc cggctggatc 181 tgctactgga catgccccct gtgtcctatg atgtccagct gctgcattca tggaacaaca 241 acgaccgatc gctcaatgtc tttgtgaagg aggacgacaa gctcatcttt caccggcatc 301 cggtggccca gagcacggac gctatcaggg gcaaagtcgg gtatacccgt gggctgcacg 361 tgtggcagat cacgtgggcc atgagacagc ggggCACACA CGCCGTGGTG GGGGTGGCGA 421 CGGCAGACGC CCCCCTGCAC TCTGTCGGGT ACACAACCCT CGTGGGGAAT AACCACGAGT 481 CCTGGGGCTG GGACTTGGGG CGCAACCGGC TCTACCACGA TGGCAAGAAC CAGCCAAGCA 541 AAACATACCC AGCCTTTCTG GAACCAGATG AGACATTCAT TGTCCCTGAC TCCTTCCTGG 601 TAGCCCTGGA CATGGACGAC GGGACTCTGA GCTTCATTGT GGATGGACAG TACATGGGAG 661 TGGCTTTTCG GGGACTCAAG GGCAAAAAAC TGTATCCTGT AGTGAGTGCC GTCTGGGGCC 721 ACTGTGAGAT CCGAATGCGC TACTTGAACG GACTCGATCC CGAGCCGCTG CCGCTCATGG 781 ATTTGTGCCG TCGCTCGGTG CGCCTGGCCC TGGGGAGGGA GCGCCTGGGG GAGATCCACA 841 CGCTGCCGCT GCCGGCTTCC CTCAAGGCCT ACCTCCTCTA CCAGTGCCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA CTCTACCGGT GATGCGTCAG TCGTACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt