Construct: ORF TRCN0000481187
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000360.1_s317c1
- Derived from:
- ccsbBroadEn_10942
- DNA Barcode:
- GGATGAGACTCAACTGCGACAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAG3 (3902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481187
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3902 | LAG3 | lymphocyte activating 3 | XM_011520956.1 | 80.1% | 79.7% | (many diffs) |
| 2 | human | 3902 | LAG3 | lymphocyte activating 3 | NM_002286.6 | 68.1% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggaggctcag ttcctgggct tgctgtttct gcagccgctt tgggtggctc 121 cagtgaagcc tctccagcca ggggctgagg tcccggtggt gtgggcccag gagggggctc 181 ctgcccagct cccctgcagc cccacaatcc ccctccagga tctcagcctt ctgcgaagag 241 caggggtcac ttggcagcat cagccagaca gtggcccgcc cgctgccgcc cccggccatc 301 ccctggcccc cggccctcac ccggcggcgc cctcctcctg ggggcccagg ccccgccgct 361 acacggtgct gagcgtgggt cccggaggcc tgcgcagcgg gaggctgccc ctgcagcccc 421 gcgtccagct ggatgagcgc ggccggcagc gcggggactt ctcgctatgg ctgcgcccag 481 cccggcgcgc ggacgccggc gagtaccgcg ccgcggtgca cctcagggac cgcgccctct 541 cctgccgcct ccgtctgcgc ctgggccagg cctcgatgac tgccagcccc ccaggatctc 601 tcagagcctc cgactgggtc attttgaact gctccttcag ccgccctgac cgcccagcct 661 ctgtgcattg gttccggaac cggggccagg gccgagtccc tgtccgggag tccccccatc 721 accacttagc gGAAAGCTTC CTCTTCCTGC CCCAAGTCAG CCCCATGGAC TCTGGGCCCT 781 GGGGCTGCAT CCTCACCTAC AGAGATGGCT TCAACGTCTC CATCATGTAT AACCTCACTG 841 TTCTGGGTCT GGAGCCCCCA ACTCCCTTGA CAGTGTACGC TGGAGCAGGT TCCAGGGTGG 901 GGCTGCCCTG CCGCCTGCCT GCTGGTGTGG GGACCCGGTC TTTCCTCACT GCCAAGTGGA 961 CTCCTCCTGG GGGAGGCCCT GACCTCCTGG TGACTGGAGA CAATGGCGAC TTTACCCTTC 1021 GACTAGAGGA TGTGAGCCAG GCCCAGGCTG GGACCTACAC CTGCCATATC CATCTGCAGG 1081 AACAGCAGCT CAATGCCACT GTCACATTGG CAATCATCAC AGGTCAGCCT CAGGTGGGAA 1141 AGGAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGATGAGAC TCAACTGCGA CAGGCACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt