Construct: ORF TRCN0000481198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010502.3_s317c1
- Derived from:
- ccsbBroadEn_14994
- DNA Barcode:
- GTGCGACTGAGCTTGCTCAAAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TWF2 (11344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11344 | TWF2 | twinfilin actin binding pro... | NM_007284.4 | 100% | 100% | |
| 2 | mouse | 23999 | Twf2 | twinfilin actin binding pro... | NM_011876.3 | 87.9% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcaccaaacg ggcatccacg ccacggaaga gctgaaggaa ttctttgcca 121 aggcacgggc tggctctgtg cggctcatca aggttgtgat tgaggacgag cagctcgtgc 181 tgggtgcctc gcaggagcca gtaggccgct gggatcagga ctatgacagg gccgtgctgc 241 cactgctgga cgcccagcag ccctgctacc tgctctaccg cctcgactca cagaatgctc 301 agggcttcga atggctcttc ctcgcctggt cgcctgataa ctcccccgtg cggctgaaga 361 tgctgtacgc ggccacgcgg gccacagtga aaaaggagtt tggaggtggc cacatcaagg 421 atgagctctt cgggactgtg aaggatgacc tctcttttgc tgggtaccag aaacacctgt 481 cgtcctgtgc ggcacctgcc ccgctgacct cggctgagag agagctccag cagatccgca 541 ttaacgaggt gaagacagag atcagtgtgg aaagcaagca ccagaccctg cagggcctcg 601 ccttccccct gcagcctgag gcccagcggg cactccagca gctcaagcag aaaatggtca 661 actacatcca gatgaagctg gaccTAGAGC GGGAAACCAT TGAGCTGGTG CACACAGAGC 721 CCACGGATGT GGCCCAGCTG CCCTCCCGGG TGCCCCGAGA TGCTGCCCGC TACCACTTCT 781 TCCTCTACAA GCACACCCAT GAGGGCGACC CCCTTGAGTC TGTAGTGTTC ATCTACTCCA 841 TGCCGGGGTA CAAGTGCAGC ATCAAGGAGC GAATGCTCTA CTCCAGCTGC AAGAGCCGCC 901 TCCTCGACTC CGTGGAGCAG GACTTCCATC TGGAGATCGC CAAGAAAATT GAGATTGGCG 961 ATGGGGCAGA GCTGACGGCA GAGTTCCTCT ACGACGAGGT GCACCCCAAG CAACACGCCT 1021 TCAAGCAGGC CTTCGCCAAG CCCAAGGGCC CAGGGGGCAA GCGGGGCCAT AAGCGCCTCA 1081 TCCGCGGCCC GGGTGAAAAT GGGGATGACA GCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 1261 GCGACTGAGC TTGCTCAAAA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt