Construct: ORF TRCN0000481236
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000488.1_s317c1
- Derived from:
- ccsbBroadEn_04162
- DNA Barcode:
- GCTTTATCACAAGTGAGCTTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD53 (79998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481236
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79998 | ANKRD53 | ankyrin repeat domain 53 | NM_024933.4 | 100% | 100% | |
| 2 | human | 79998 | ANKRD53 | ankyrin repeat domain 53 | NM_001115116.2 | 60.7% | 54.1% | (many diffs) |
| 3 | human | 79998 | ANKRD53 | ankyrin repeat domain 53 | NM_001369683.1 | 54.4% | 48.1% | (many diffs) |
| 4 | human | 79998 | ANKRD53 | ankyrin repeat domain 53 | XM_011533114.3 | 50.5% | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccgcgggc agcaccgctc ggcgggcggg ctccggaagc tggcactcag 121 aaaggggaga agggagaggt gctcggccgc agccaactcc aagtggctcc atgcagcagg 181 cgaacaaagt ctccttgaag gccacctgga ctgacgcgga gtccaagcag cccagccagc 241 ccctgcccga cctcgcagac cacctcagtg cgcaggcgac tgccctcgcc aggccgcgcc 301 gccctgcctc gctcaccccg ccccgcgctg accccagccc cagcaaggag tccgaccaga 361 cggcaatcga ccagacggcg atcgggagct actaccagct gttcgcagcg gctgtgggca 421 acgtggaatg gctgcgattc tgtctgaacc agagcctcag ggaaatcccc accgacgaca 481 agggcttcac tgccatccac ttcgccgccc aatggggcaa gcttgcatgc ctgcaggtcc 541 tggtagagga gtacaagttt cccgtggacc tgctgaccaa caatagccag acacccctgc 601 acctcgtcat ccacagggac aacaccaccg tggccctccc ctgcatctac tacctgctgg 661 agaaaggcgc agacctcaat gcTCAGACAT GCAACGGCTC CACGCCCCTG CACCTGGCAG 721 CCCGTGACGG CTTGCTGGAC TGTGTGAAGG TCCTGGTGCA GAGTGGCGCC AACGTCCATG 781 CCCAAGATGC CATGGGCTAC AAACCCATTG ACTTCTGCAA AATATGGAAC CACCGTGCCT 841 GTGCCCGGTT CTTGAAGGAT GCCATGTGGA AAAAGGACAA GAAGGACTTT GCCCGTGAGA 901 TGACGAAAAT GAAGATGTTC AAGAGCCAGC TGACCCTCAT GGAGCACAAC TACCTGATTG 961 AGTATCAAGG TCAAGGATGC AGTGTGCACT TCCCATTTGC CTTTTCCCCC ATCACACCAG 1021 AGACTCTCCT ATGGCAGGAC ATTTCTCTAC TATCAGACTG TGGATTCCTT TGGAGAAGAA 1081 GGGAATTGTC TTTCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGGCTTTA TATATCTTGT GGAAAGGACG AGCTTTATCA CAAGTGAGCT 1261 TTTTCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt