Construct: ORF TRCN0000481273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015016.1_s317c1
- Derived from:
- ccsbBroadEn_09883
- DNA Barcode:
- CTTGCCTTATGGGCTGGATTGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNC5CL (222643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222643 | UNC5CL | unc-5 family C-terminal like | NM_173561.3 | 99.9% | 99.8% | 1294A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1620
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg cccccaggag agttcattcc aaccctccca gttcctactg ctggtggggg 121 tcccagtggc aagtgtcctc cttctggccc aatgccttcg atggcactgc cctagaaggc 181 tgctgggggc ctgctggaca ctgaatggtc aagaggaacc agtgtcccag cctacccccc 241 aactagaaaa tgaggtctca aggcagcacc tgccagccac actgccagag atggttgcct 301 tctaccagga gctacacaca cccactcaag gccagaccat ggtccgccag ttgatgcaca 361 aactgttggt gttttcggct cgagaggtgg atcaccgcgg cggttgcctg atgctccagg 421 atacaggcat ctccttgctc atcccaccag gtgctgtggc tgtgggccgc caggagcggg 481 tgtctttgat cctggtgtgg gacctgtcgg acgccccatc gctgtcccaa gcccaggggc 541 tggtaagccc tgtggtggca tgtggccccc atggggcctc cttcctgaag ccttgcactc 601 tcacgttcaa acactgtgcc gagcagccca gccatgctcg cacctacagc agcaacacta 661 ccctgctgga tgccaaggta tggaggcccc tggggcggcc gggggcccac gcctcccggg 721 atgagtgtcg catccacctc tcccacttca gcctctacac ctgtgtgctg gaggcacctg 781 tggggcgcga agcccgcaaa tggctgcagc tggccgtatt ctgctcaccg ctggtgccag 841 gacagtccca tctgcaactg cgtatctact tcctcaacaa cacgccctgc gccctgcagt 901 gggcactgac caacgagcag ccccatggtg ggcgcctgcg tgggccctgc cagctcttcg 961 acttcaatgg ggctaggggc gaccagtgcc tgaagctcac gtacatctca gagggttggg 1021 agaatgtgga tgacagcagt tgccagctgg ttccccatct ccacatctgg catggaaagt 1081 gccccttccg ctccttctgc ttccggagaa aagcagccga tgagaatgag gactgttcag 1141 cactaaccaa tgagatcatt gtcaccatgc acaccttcca ggatggcttg gagaccaagt 1201 atatggaaat ccTCAGATTC CAGGCATCAG AGGAGGAATC CTGGGCAGCG CCACCACCTG 1261 TTTCCCAGCC GCCCCCATGC AATAGGCTGC CCCCAGAGCT CTTTGAGCAG CTGCGGATGT 1321 TATTGGAGCC AAACAGCATC ACCGGCAATG ACTGGCGCGG ACTGGCCTCC CACCTGGGGC 1381 TTTGCGGCAT GAAGATCCGG TTCCTGTCCT GCCAGCGCAG CCCCGCAGCG GCCATCCTGG 1441 AGTTGTTTGA GGAGCAGAAC GGCAGCCTGC AGGAGCTGCA CTACCTCATG ACCGTCATGG 1501 AGCGGCTAGA CTGCGCCTCC GCCATCCAGA ACTACCTGAG TGGGACACAC GGCGGCAGCC 1561 CAGGCCCCGA GCGCGGGGGC GCCCGGGATA ACCAGGGCCT GGAGCTGGAC GAGAAGCTCT 1621 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGACTTGC CTTATGGGCT GGATTGTGTA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt