Construct: ORF TRCN0000481282
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009091.1_s317c1
- Derived from:
- ccsbBroadEn_03354
- DNA Barcode:
- ACCTCGTGAGCACCCTGGCCTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS23 (51649)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481282
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51649 | MRPS23 | mitochondrial ribosomal pro... | NM_016070.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggcagccgg ctggaaaccg tagggagcat cttctctcgg actcgggacc 121 tggttcgggc cggggtgctg aaggagaagc ccctgtggtt tgacgtatat gacgcctttc 181 ccccgcTGAG GGAGCCCGTC TTCCAAAGGC CTCGAGTGCG ATATGGCAAA GCCAAAGCTC 241 CCATCCAAGA CATCTGGTAC CACGAGGATC GGATTAGAGC GAAGTTTTAT TCAGTGTATG 301 GGTCTGGTCA AAGAGCTTTT GATCTATTCA ATCCAAACTT CAAGTCTACC TGTCAACGGT 361 TTGTGGAGAA GTACACTGAG CTACAGAAAC TTGGAGAAAC AGATGAAGAG AAGTTATTTG 421 TGGAAACAGG GAAGGCTTTA TTGGCAGAAG GTGTCATTTT AAGACGAGTA GGCGAAGCAA 481 GGACTCAACA CGGAGGTAGT CACGTTTCCC GGAAATCCGA ACACTTGAGT GTCAGACCAC 541 AGACTGCGTT GGAAGAAAAC GAGACTCAGA AAGAAGTTCC ACAGGACCAG CATTTGGAGG 601 CACCTGCAGA CCAGTCGAAA GGTCTCTTGC CTCCCTGCCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AACCTCGTGA GCACCCTGGC CTCGAACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt