Construct: ORF TRCN0000481336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007235.1_s317c1
- Derived from:
- ccsbBroadEn_05443
- DNA Barcode:
- GATTCCTCTGTAGCATGTTTTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR5AP2 (338675)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481336
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338675 | OR5AP2 | olfactory receptor family 5... | NM_001002925.1 | 100% | 100% | |
| 2 | mouse | 258573 | Olfr1020 | olfactory receptor 1020 | NM_146580.2 | 80.3% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1017
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagacttatg aaagaggttc gaggcagaaa tcaaacagaa gtaacagaat 121 ttctcctctt aggactttcc gacaatccag atctacaagg agtcctcttt gcattgtttc 181 tgttgatcta tatggcaaac atggtgggca atttggggat gattgtattg attaagattg 241 atctctgtct ccacaccccc atgtatttct ttctcagtag cctctctttt gtagatgcct 301 cttactcttc ttccgtcact cccaagatgc tggtgaacct catggctgag aataaggcca 361 tttcttttca tggatgtgct gcccagttct acttctttgg ctccttcctg gggactgagt 421 gcttcctgtt ggccatgatg gcatatgacc gctatgcagc catttggaac cccctgctct 481 acccagttct cgtgtctggg agaatttgct ttttgctaat agctacctcc ttcttagcag 541 gttgtggaaa tgcagccata catacaggga tgacttttag gttgtccttt tgtggttcta 601 ataggatcaa ccatttctac tgtgacaccc cgccactgct caaactctct tgctctgata 661 cccacttcaa tggCATTGTG ATCATGGCAT TCTCAAGTTT TATTGTCATC AGCTGTGTTA 721 TGATTGTCCT CATTTCCTAC CTGTGTATCT TCATTGCCGT CTTGAAGATG CCTTCGTTAG 781 AGGGCAGGCA CAAAGCCTTC TCCACCTGTG CCTCTTACCT CATGGCTGTC ACCATATTCT 841 TTGGAACAAT CCTCTTCATG TACTTGCGCC CTACATCTAG CTACTCAATG GAGCAAGACA 901 AGGTTGTCTC TGTCTTTTAT ACAGTAATAA TCCCTGTGCT AAATCCCCTC ATCTATAGTT 961 TAAAAAATAA GGATGTAAAA AAGGCCCTAA AGAAGATCTT ATGGAAACAC ATCTTGTTGC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GAGATTCCTC TGTAGCATGT TTTCGAACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt