Construct: ORF TRCN0000481355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012107.1_s317c1
- Derived from:
- ccsbBroadEn_02325
- DNA Barcode:
- AGCTGTGTAACACAAGGTACGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPTN (10133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10133 | OPTN | optineurin | NM_001008211.1 | 100% | 100% | |
| 2 | human | 10133 | OPTN | optineurin | NM_001008212.2 | 100% | 100% | |
| 3 | human | 10133 | OPTN | optineurin | NM_001008213.1 | 100% | 100% | |
| 4 | human | 10133 | OPTN | optineurin | NM_021980.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1797
- ORF length:
- 1731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccatcaacct ctcagctgcc tcactgaaaa ggaggacagc cccagtgaaa 121 gcacaggaaa tggacccccc cacctggccc acccaaacct ggacacgttt accccggagg 181 agctgctgca gcagatgaaa gagctcctga ccgagaacca ccagctgaaa gaagccatga 241 agctaaataa tcaagccatg aaagggagat ttgaggagct ttcggcctgg acagagaaac 301 agaaggaaga acgccagttt tttgagatac agagcaaaga agcaaaagag cgtctaatgg 361 ccttgagtca tgagaatgag aaattgaagg aagagcttgg aaaactaaaa gggaaatcag 421 aaaggtcatc tgaggacccc actgatgact ccaggcttcc cagggccgaa gcggagcagg 481 aaaaggacca gctcaggacc caggtggtga ggctacaagc agagaaggca gacctgttgg 541 gcatcgtgtc tgaactgcag ctcaagctga actccagcgg ctcctcagaa gattcctttg 601 ttgaaattag gatggctgaa ggagaagcag aagggtcagt aaaagaaatc aagcatagtc 661 ctgggcccac gagaacagtc tccactggca cggcattgtc taaatatagg agcagatctg 721 cagatggggc caagaattac ttcgaacatg aggagttaac tgtgagccag ctcctgctgt 781 gcctaaggga agggaatcag aaggtggaga gacttgaagt tgcactcaag gaggccaaag 841 aaagagtttc agattttgaa aagaaaacaa gtaatcgttc tgagattgaa acccagacag 901 aggggagcac agagaaagag aatgatgaag agaaaggccc ggagactgtt ggaagcgaag 961 tggaagcact gaacctccag gtgacatctc tgtttaagga gcttcaagag gctcatacaa 1021 aactcagcga agctgagcta atgaagaaga gacttcaaga aaagtgtcag gcccttgaaa 1081 ggaaaaattc tgcaattcca tcagagttga atgaaaagca agagcttgtt tatactaaca 1141 aaaagttaga gctacaagtg gaaagcatgc tatcagaaat caaaatggaa caggctaaaa 1201 cagaggatga aaagtccaaa ttaactgtgc tacagatgac acacaacaag cttcttcaag 1261 aacataataa tgcattgaaa acaattgagg aactaaCAAG AAAAGAGTCA GAAAAAGTGG 1321 ACAGGGCAGT GCTGAAGGAA CTGAGTGAAA AACTGGAACT GGCAGAGAAG GCTCTGGCTT 1381 CCAAACAGCT GCAAATGGAT GAAATGAAGC AAACCATTGC CAAGCAGGAA GAGGACCTGG 1441 AAACCATGAC CATCCTCAGG GCTCAGATGG AAGTTTACTG TTCTGATTTT CATGCTGAAA 1501 GAGCAGCGAG AGAGAAAATT CATGAGGAAA AGGAGCAACT GGCATTGCAG CTGGCAGTTC 1561 TGCTGAAAGA GAATGATGCT TTCGAAGACG GAGGCAGGCA GTCCTTGATG GAGATGCAGA 1621 GTCGTCATGG GGCGAGAACA AGTGACTCTG ACCAGCAGGC TTACCTTGTT CAAAGAGGAG 1681 CTGAGGACAG GGACTGGCGG CAACAGCGGA ATATTCCGAT TCATTCCTGC CCCAAGTGTG 1741 GAGAGGTTCT GCCTGACATA GACACGTTAC AGATTCACGT GATGGATTGC ATCATTTACC 1801 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1861 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1921 ATATATCTTG TGGAAAGGAC GAAGCTGTGT AACACAAGGT ACGTTGACGC GTTAAGTCga 1981 caatcaacct ctggattaca aaatttgtga aagatt