Construct: ORF TRCN0000481455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004252.1_s317c1
- Derived from:
- ccsbBroadEn_14500
- DNA Barcode:
- TCCTACTTGTTACAAAGGCCCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR1915HG (399726)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 399726 | MIR1915HG | MIR1915 host gene | NR_160800.1 | 10.7% | 1_446del;855_3799del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCACCAT GCAATCGCGG GAGCCCTCAG GGTGGCGCAC GGCGGAGAGG CGACGCGGGT 121 GGCGCTGCAG AGGCGTCCCC ACCCCCTCCG GGGACCGGGG CCCCGGAGCT GCCAGGCCCG 181 CTGCACGCGG CGGCGCGGGA GAAACCACGG GGCAGCAGCG CCCCCTGCAG GTGCCGGGCG 241 CCTCCGCAGC CGCTGCGCGG ACCCGACTCT TAAGGTGGCA CCACCGGGTT CCCAGCCCGC 301 GCGCAACCCG CAGCCCTGGC TCCATCCGCC GCACTTCTCC CTGTAGCGGC GGGCCTGACC 361 GTCCGGAACG GCCGGAGTGT GCTGATGCCT GCTGCTATCT GCTTGATCCC TTCACCCTAC 421 CTCTCATCCA GGACTTCTTC CGCGGATGCG CAGCTAGCGA TTTCGACAGG CGAGATTTGC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GATCCTACTT GTTACAAAGG CCCAGTACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt