Construct: ORF TRCN0000481531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017909.2_s317c1
- Derived from:
- ccsbBroadEn_06161
- DNA Barcode:
- AGTCCTAAATGACATCCTTCAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENO2 (2026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481531
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2026 | ENO2 | enolase 2 | NM_001975.3 | 99.9% | 100% | 1047G>T |
| 2 | mouse | 13807 | Eno2 | enolase 2, gamma neuronal | NM_013509.3 | 91.6% | 98.6% | (many diffs) |
| 3 | mouse | 13807 | Eno2 | enolase 2, gamma neuronal | XM_006505503.3 | 91.6% | 98.6% | (many diffs) |
| 4 | mouse | 13807 | Eno2 | enolase 2, gamma neuronal | XM_006505504.2 | 91.6% | 98.6% | (many diffs) |
| 5 | mouse | 13807 | Eno2 | enolase 2, gamma neuronal | NM_001302642.1 | 66.2% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catagagaag atctgggccc gggagatcct ggactcccgc gggaacccca 121 cagtggaggt ggatctctat actgccaaag gtcttttccg ggctgcagtg cccagtggag 181 cctctacggg catctatgag gccctggagc tgagggatgg agacaaacag cgttacttag 241 gcaaaggtgt cctgaaggca gtggaccaca tcaactccac catcgcgcca gccctcatca 301 gctcaggtct ctctgtggtg gagcaagaga aactggacaa cctgatgctg gagttggatg 361 ggactgagaa caaatccaag tttggggcca atgccatcct gggtgtgtct ctggccgtgt 421 gtaaggcagg ggcagctgag cgggaactgc ccctgtatcg ccacattgct cagctggccg 481 ggaactcaga cctcatcctg cctgtgccgg ccttcaacgt gatcaatggt ggctctcatg 541 ctggcaacaa gctggccatg caggagttca tgatcctccc agtgggagct gagagctttc 601 gggatgccat gcgactaggt gcagaggtct accatacact caagggagtc atcaaggaca 661 aatacggcaa ggatgccacc aatgtggggg atgaaggtgg ctttgccccc aatatcctgg 721 agaacagtga agccttggag ctggtgaagg aagccatcga caaggctggc tacacggaaa 781 agatcgttat tggcatggat gttgctgcct cagagtttta tcgtgatggc aaatatgact 841 tggacttcaa gtctcccact gatccttccc gatacatcac tggggaccag ctgggggcac 901 tctaccagga ctttgtcagg gacTATCCTG TGGTCTCCAT TGAGGACCCA TTTGACCAGG 961 ATGATTGGGC TGCCTGGTCC AAGTTCACAG CCAATGTAGG GATCCAGATT GTGGGTGATG 1021 ACCTGACAGT GACCAACCCA AAACGTATTG AGCGGGCAGT GGAAGAAAAG GCCTGCAACT 1081 GTCTGCTGCT CAAGGTCAAC CAGATCGGCT CTGTCACTGA AGCCATCCAA GCGTGCAAGC 1141 TGGCCCAGGA GAATGGCTGG GGGGTCATGG TGAGTCATCG CTCAGGAGAG ACTGAGGACA 1201 CATTCATTGC TGACCTGGTG GTGGGGCTGT GCACAGGCCA GATCAAGACT GGTGCCCCGT 1261 GCCGTTCTGA ACGTCTGGCT AAATACAACC AGCTCATGAG AATTGAGGAA GAGCTGGGGG 1321 ATGAAGCTCG CTTTGCCGGA CATAACTTCC GTAATCCCAG TGTGCTGTGC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAAGTCCTA AATGACATCC TTCAAGCACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt