Construct: ORF TRCN0000481539

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007278.2_s317c1
Derived from:
ccsbBroadEn_07063
DNA Barcode:
CCGCATCAACTCAAATCGATCAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGM3 (7053)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481539
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7053 TGM3 transglutaminase 3 NM_003245.4 99.9% 99.7% 38C>A;1960G>C
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2148
ORF length:
2079
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctgctcta ggagtccaga gtatcaactg gcagaaggcc ttcaaccgac
121aagcgcatca cacagacaag ttctccagcc aggagctcat cttgcggaga ggccaaaact
181tccaggtctt aatgatcatg aacaaaggcc ttggctctaa cgaaagactg gagttcattg
241tctccacagg gccttacccc tcagagtcgg ccatgacgaa ggctgtgttt ccactctcca
301atggcagtag tggtggctgg agtgcggtgc ttcaggccag caatggcaat actctgacta
361tcagcatctc cagtcctgcc agcgcaccca taggacggta cacaatggcc ctccagatct
421tctcccaggg cggcatctcc tctgtgaaac ttgggacgtt catactgctt tttaacccct
481ggctgaatgt ggatagcgtc tttatgggta accacgctga gagagaagag tatgttcagg
541aagatgccgg catcatcttt gtgggaagca caaaccgaat tggcatgatt ggctggaact
601ttggacagtt tgaagaagac attctcagca tctgcctctc aatcttggat aggagtctga
661atttccgccg tgacgctgct actgatgtgg ccagcagaaa tgaccccaaa tacgttggcc
721gggtgctgag tgccatgatc aatagcaatg atgacaatgg tgtgcttgct gggaattgga
781gcggcactta caccggtggc cgggacccaa ggagctggaa cggcagcgtg gagatcctca
841aaaattggaa aaaatctggc ttcagcccag tccgatatgg ccagtgctgg gtctttgctg
901ggaccctcaa cacagcgctg cggtctttgg ggattccttc ccgggtgatc accaacttca
961actcagctca tgacacagac cgaaatctca gtgtggatgt gtactacgac cccatgggaa
1021accccctgga caagggtagt gatagcgtat ggaatttcca tgtctggaat gaaggctggt
1081ttgtgaggtc tgacctgggc ccctcgtacg gtggatggca ggtgttggat gctaccccgc
1141aggaaagaag ccaaggggtg ttccagtgcg gccccgcttc ggtcattggt gttcgagagg
1201gtgatgtgca gctgaacttc gacatgccct ttatcttcgc ggaggttaat gccgaccgca
1261tcacctggct gtacgacaac accactggca aacagtggaa gaattccgtg aacagtcaca
1321ccattggcag gtacatcagc accaaggcgg tgggcagcaa tgctcgcatg gacgtcacgg
1381acaagtacaa gtacccagaa ggctctgacc aggaaagaca agtgttccaa aaggctttgg
1441ggaaacttaa acccaacacg ccatttgccg cgacgtcttc aatgggtttg gaaacagagg
1501aacaggagcc cagcatcatc gggaagctga aggtcgctgg catgctggca gtaggcaaag
1561aagtcaacct ggtcctactg ctcaaaaacc tgagcaggga tacgaagaca gtgacagtga
1621acatgacagc ctggaccatc atctacaacg gcacgcttgt acatgaagtg tggaaggact
1681ctgccacaat gtccctggac cctgaggaag aggcagaaca TCCCATAAAG ATCTCGTACG
1741CTCAGTATGA GAAGTACCTG AAGTCAGACA ACATGATCCG GATCACAGCG GTGTGCAAGG
1801TCCCAGATGA GTCTGAGGTG GTGGTGGAGC GGGACATCAT CCTGGACAAC CCCACCTTGA
1861CCCTGGAGGT GCTGAACGAG GCTCGTGTGC GGAAGCCTGT GAACGTGCAG ATGCTCTTCT
1921CCAATCCACT GGATGAGCCG GTGAGGGACT GCGTGCTGAT GGTGGAGGGA AGCGGCCTGC
1981TGTTGGGTAA CCTGAAGATC GACGTGCCGA CCCTAGGGCC CAAGGAGCGG TCCCGGGTCC
2041GTTTTGATAT CCTGCCCTCC CGGAGTGGCA CCAAGCAACT GCTCGCCGAC TTCTCCTGCA
2101ACAAGTTCCC TGCAATCAAG GCCATGTTGT CCATCGATGT AGCCGAATTG CCAACTTTCT
2161TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2221CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2281GTGGAAAGGA CGACCGCATC AACTCAAATC GATCAGTACG CGTTAAGTCg acaatcaacc
2341tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)