Construct: ORF TRCN0000481570
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013175.1_s317c1
- Derived from:
- ccsbBroadEn_11767
- DNA Barcode:
- CCAGTCGGGTCCAAGCTGCGTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP45 (25790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481570
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25790 | CFAP45 | cilia and flagella associat... | NM_012337.3 | 84.5% | 84.5% | 1_255del |
| 2 | mouse | 71870 | Cfap45 | cilia and flagella associat... | NM_027972.1 | 72.7% | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1467
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtccgagaa ctcattgttc ccacagagga tccctccggg gagtccctaa 121 tcatcagccc tgaggagttt gagcgaatca aatgggcatc ccatgtcctg accagagaag 181 aacttgaggc cagggaccag gccttcaaga aggagaagga agccaccatg gatgcagtga 241 tgacacgaaa gaagatcatg aaacagaagg agatggtgtg gaacaacaac aagaagctca 301 gtgacctgga ggaggtggcc aaggaacggg cccagaacct cctgcagaga gccaacaagc 361 tgcggatgga gcaggaggag gagctcaagg acatgagcaa gattatcctc aatgctaagt 421 gccatgccat ccgggatgcc caaatcctgg agaagcagca gatccaaaaa gaactggaca 481 cagaagagaa gcggttggat cagatgatgg aagtggagcg gcagaaatcc attcaaaggc 541 aggaggaact ggagaggaag aggagggagg aaagaattag aggaaggcgg caaattgtgg 601 aacagatgga aaagaaccag gaggagcgat cgctgcttgc tgagcagcgg gagcaggaga 661 aggagcagat gctggaatat atggaacagc tccaagagga agatctaaag gacatggaac 721 gaaggcagca acaaaaactg aagatgcaag ctgagattaa gcgcatcaat gatgaaaacc 781 agaaacagaa agcagaactg ctggctcagg agaagctggc agaccagatg gtgatggagt 841 ttaccaagaa gaagatggct cgagaagcag agtttgaggc tgagcaggag agaatccgga 901 gggagaaaga gaaggagatc gcacgcttga gggccatgca ggagaaggcc caggattacc 961 aggcagaaca ggatgccttg cgggccaagc gcaaccagga ggttgCAGAC AGAGAGTGGC 1021 GCAGAAAGGA AAAGGAAAAT GCGCGGAAGA AGATGGAAAC AGAGGCTGAG CTGCGAAAAA 1081 GTCGGCTCGA ACAGGTGGCT TTCAAGGAGC ACGCTCTGGC TGTTCAGGTG CAACGGGACC 1141 GGGATGAGTT CGAGAGGATT CTTCGGGCTC AGAGAGAACA GATTGAGAAG GAGCGGCTGG 1201 AGGAGGAGAA AAAGGCCACA GGGCGCTTAC AGCATGCCAA TGAGCTCCGG CGCCAGGTGC 1261 GCGAGAACCA GCAGAAGGAA GTGCAGAACC GGATTGCCAC CTTTGAGGAG GGCCGGCGCC 1321 TCAAAGAGGA GGCCCAGAAA CGCCGTGAGC GCATCGATGA GATCAAGAGG AAAAAGCTTG 1381 AAGAGCTGAG AGCCACTGGC CTTCCCGAGA AGTACTGCAT TGAAGCTGAG CGCAAAGCTA 1441 ACATCCTGCC AGCTACCTCT GTGAACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCAGTCGG 1621 GTCCAAGCTG CGTTGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt