Construct: ORF TRCN0000481588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009466.1_s317c1
- Derived from:
- ccsbBroadEn_05300
- DNA Barcode:
- CAGCATCGACAGTTCCTATACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC41A1 (254428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481588
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254428 | SLC41A1 | solute carrier family 41 me... | NM_173854.6 | 100% | 100% | |
| 2 | human | 254428 | SLC41A1 | solute carrier family 41 me... | XM_005245069.2 | 100% | 100% | |
| 3 | mouse | 98396 | Slc41a1 | solute carrier family 41, m... | NM_173865.3 | 91.6% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1608
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcctctaag ccagagccga aggacgtcca ccaactgaac gggactggcc 121 cttctgcctc tccctgctct tcagatggcc cagggagaga gcccttggct gggacctcag 181 agttcctggg gcctgatggg gctggggtag aggtggtgat tgagtctcgg gccaacgcca 241 agggggttcg ggaggaggac gccctgctgg agaacgggag ccagagcaac gaaagtgacg 301 acgtcagcac agaccgtggc cctgcgccac cttccccgct caaggagacc tccttttcca 361 tcgggctgca agtactgttt ccattcctcc tggcaggctt tgggaccgtg gctgctggca 421 tggtgttgga catcgtgcag cactgggaag tcttccagaa ggtgacagag gtcttcatcc 481 tagtgcctgc gctgctgggg ctcaaaggga acctggaaat gaccctggca tcaaggcttt 541 ccactgcagc caacattgga cacatggaca cacccaagga gctctggcgg atgatcactg 601 ggaacatggc cctcatccag gtgcaggcca cggtggtggg cttcctggcg tccatcgcag 661 ccgtcgtctt tggctggatc cctgatggcc acttcagtat tccgcacgcc ttcctgctct 721 gtgctagcag cgtggccaca gccttcattg cctccctggt actgggtatg atcatgattg 781 gagtcatcat tggctctcgc aagattggga tcaacccaga caacgtggcc acacccattg 841 ctgccagcct gggcgacctc atcaccttgg cgctgctctc aggcatcagc tggggactct 901 acctggaact gaatcactgg cgatacatct acccactggt gtgtgctttc tttgtggccc 961 tgctgcctgt ctgggtggtg ctggcccgac gaagtccagc cacaagggag gtgttgtact 1021 cgggctggga gcctgttatc attgccatgg ccatcagcag tgtgggaggc ctcatcttgg 1081 acaagactgt ctcagacccc aactttgctg ggatggctgt cttcacgcct gtgattaatg 1141 gtgttggggg caatctggtg gcagtgcagg CCAGCCGCAT CTCCACCTTC CTGCACATGA 1201 ATGGAATGCC CGGAGAGAAC TCTGAGCAAG CTCCTCGCCG CTGTCCCAGT CCTTGTACCA 1261 CCTTCTTCAG CCCTGATGTG AATTCTCGCT CAGCCCGGGT CCTCTTCCTC CTCGTGGTCC 1321 CAGGACACCT GGTGTTCCTC TACACCATCA GCTGTATGCA GGGCGGGCAC ACCACCCTCA 1381 CACTCATCTT CATCATCTTC TATATGACAG CTGCACTGCT CCAGGTGCTG ATTCTCCTGT 1441 ACATCGCAGA CTGGATGGTG CACTGGATGT GGGGCCGGGG CCTGGACCCG GACAACTTCT 1501 CCATCCCATA CTTGACTGCT CTGGGGGACC TGCTTGGCAC TGGGCTCCTA GCACTCAGCT 1561 TCCATGTTCT CTGGCTCATA GGGGACCGAG ACACGGATGT CGGGGACTTG CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGACAGCATC GACAGTTCCT ATACCGCACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt