Construct: ORF TRCN0000481652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000206.1_s317c1
- Derived from:
- ccsbBroadEn_13791
- DNA Barcode:
- CCAGATATTACGTGCCCGGTGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481652
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 50.9% | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggaccgtt ctcctcctcg gcctcctctc tcactgcaca gactctgtgg 121 cctcctatgt gttgactcag ccaccctcgg tgtcagtggc cccaggaaag acggccagga 181 ttacctgtgg ggcagacaac attggagcta aaagtgtgca ctggtatcaa cagaagaccg 241 accaggcccc tgttctggtc gtccatgacg acaacgaccg gccctcaggg atccctgagc 301 ggttctctgg ctccaattct gggaacacgg ccaccctgag catcagcagg gtcgagcccg 361 gggatgaggc cgactatttt tgtcaggtgt gggataatag tggtggtcag ctttGGATGT 421 TCGGCGGAGG GACCAAGTTG ACCGTCCTAC GTCAGCCCAA GGCTGCCCCC TCGGTCACTC 481 TGTTCCCACC CTCCTCTGAG GAGCTTCAAG CCAACAAGGC CACACTGGTG TGTCTCATAA 541 GTGACTTCTA CCCGGGAGCC GTGACAGTGG CCTGGAAGGC AGATAGCAGC CCCGTCAAGG 601 CGGGAGTGGA GACCACCACA CCCTCCAAAC AAAGCAACAA CAAGTACGCG GCCAGCAGCT 661 ACCTGAGCCT GACGCCTGAG CAGTGGAAGT CCCACAAAAG CTACAGCTGC CAGGTCACGC 721 ATGAAGGGAG CACCGTGGAG AAGACAGTGG CCCCTACAGA ATGTTCATAC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGACCAGATA TTACGTGCCC GGTGGGGACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt