Construct: ORF TRCN0000487696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019511.1_s317c1
- DNA Barcode:
- ATGAATTATCCAACACTACAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TESK2 (10420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10420 | TESK2 | testis associated actin rem... | NM_007170.3 | 100% | 100% | |
| 2 | human | 10420 | TESK2 | testis associated actin rem... | NM_001320800.2 | 85.4% | 85.4% | 0_1ins249 |
| 3 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | NM_146151.4 | 86.7% | 87.7% | (many diffs) |
| 4 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XM_006503008.3 | 68.8% | 69.1% | (many diffs) |
| 5 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XR_001784124.1 | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1803
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcacctct agacagacta tggatcggag caaacggaat tcaattgcag 121 gatttcctcc acgtgtggag cgtcttgaag agtttgaagg aggtggtgga ggagaaggaa 181 atgtgagcca ggtgggaaga gtttggccat cttcgtatcg agctcttata agtgcctttt 241 ccagactgac gcgtttggat gatttcacct gtgaaaaaat agggtctggc ttcttttctg 301 aagtgttcaa ggtacgacac cgagcttctg gtcaggtgat ggctcttaag atgaacacat 361 tgagcagtaa ccgggcaaac atgctgaaag aagtacagct catgaataga ctctcccatc 421 ccaacatcct taggttcatg ggtgtatgtg ttcatcaagg acaattgcat gcacttacag 481 agtatatcaa ctccgggaac ctggaacagt tgctagacag taacctgcat ttgccttgga 541 ctgtgagggt aaaactggcc tatgacatag cagtgggcct cagctacctt cacttcaaag 601 gcatttttca tcgggacctc acatctaaga actgcctgat aaagagggat gagaatggtt 661 actctgcagt ggtagctgac tttggcctgg ctgagaagat ccccgatgtc agcatgggga 721 gtgagaagct ggccgtggtg ggttccccat tctggatggc acctgaggtt ctccgagatg 781 agccctataa tgaaaaggca gatgtgttct cttatggtat catcctctgc gagatcatcg 841 cccgcatcca ggccgatccg gactatcttc cccgcacaga gaatttcggg ctggactatg 901 atgctttcca gcacatggtg ggagactgtc ccccagattt tctgcaactt actttcaact 961 gctgtaacat ggatcccaaa ctgcgcccat cttttgtgga gattgggaag accctggagg 1021 aaattctgag ccgcctacag gaagaagagc aggagaggga taggaagctg cagcccacag 1081 ccaggggact cttggagaaa gcacctgggg tgaagcgact aagctcactg gatgacaaga 1141 tcccccacaa gtcaccatgc ccaagacgta ccatctggct gtctcgaagc cagtcagata 1201 tcttttcccg taagccccca cgtacagtga gtgtcttgga cccatactac cggccacgag 1261 atggtgctgc ccgcaccccc aaagtcaacc cttttagtgc tcgccaggac ctcatggggg 1321 gcaagatcaa gttttttgac ctgcccagca agtctgtcat ctctctggta tttgacctgg 1381 atgcaccagg gcccggaact atgcccctgg ctgactggca ggagcccctg gccccaccta 1441 ttcgccggtg gcgttccttg cctggttcgc ctgagttctt gcatcaagag gcttgtccat 1501 ttgtgggccg ggaagaatcg ctatctgatg ggcccccacc acgccTAAGT AGTCTCAAGT 1561 ACAGAGTTAA AGAGATCCCA CCATTCCGGG CATCTGCCCT ACCAGCTGCT CAAGCCCATG 1621 AGGCTATGGA CTGCTCCATT CTCCAGGAAG AAAATGGTTT TGGGTCCAGG CCCCAGGGGA 1681 CCAGTCCATG CCCTGCGGGT GCTTCTGAGG AGATGGAGGT AGAAGAAAGG CCAGCAGGCT 1741 CAACTCCAGC CACCTTCTCC ACCTCAGGCA TAGGCCTGCA AACCCAGGGA AAGCAGGATG 1801 GGAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1861 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1921 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATGAATT ATCCAACACT 1981 ACAATCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt