Construct: ORF TRCN0000487703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019719.1_s317c1
- DNA Barcode:
- CGGCATTTAAATAACATGTTCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFB6 (4712)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487703
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_002493.5 | 99.7% | 99.2% | 384_385insG |
| 2 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_182739.3 | 88% | 87.5% | 271_272ins45;339_340insG |
| 3 | human | 4712 | NDUFB6 | NADH:ubiquinone oxidoreduct... | NM_001199987.1 | 75.5% | 75.1% | 180_181ins93;291_292insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 462
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgacg gggtacactc cggatgagaa actgcggctg cagcagctgc 121 gagagctgag aaggcgatgg ctgaaggacc aggagctgag ccctcgggag ccggtgctgc 181 ccccacagaa gatggggcct atggagaaat tctggaataa atttttggag aataaatccc 241 cttGGAGGAA AATGGTCCAT GGGGTATACA AAAAGAGTAT CTTTGTTTTC ACTCATGTAC 301 TTGTACCTGT CTGGATTATT CATTATTACA TGAAGTATCA TGTTTCTGAA AAACCATATG 361 GCATAGTTGA AAAGAAGTCC AGAATATTCC CTGGTGATAC AATTCTGGAG ACTGGAGAAG 421 TAATTCCACC AATGAAAGAA TTTCCTGATC AACATCATGA CCCAGCTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGACGGCAT TTAAATAACA TGTTCCGAAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt