Construct: ORF TRCN0000487755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021022.1_s317c1
- DNA Barcode:
- ATGAGCTACATCCACTGTATTGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TMEM11 (8834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487755
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8834 | TMEM11 | transmembrane protein 11 | NM_003876.2 | 100% | 100% | |
| 2 | human | 8834 | TMEM11 | transmembrane protein 11 | NR_024547.1 | 34.1% | 1_443del;506_797del;1312_1688del | |
| 3 | mouse | 216821 | Tmem11 | transmembrane protein 11 | NM_173453.3 | 90.2% | 96.8% | (many diffs) |
| 4 | mouse | 216821 | Tmem11 | transmembrane protein 11 | NM_001168507.1 | 82.9% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 648
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgct tggggaagga ggcgtcttgg cccgggcagc agtggcggca 121 gcgcccgaga gagggtgagc ttgtcggcca cagactgcta cattgtgcat gagatctaca 181 atggggagaa tgcccaagac cagtttgagt acgagctgga gcaggccctg gaagcccagt 241 acaagtacat tgtgattgag cccactcgca ttggcgacga gacagcccgc tggatcaccg 301 tgggcaactg cctgcacaag acggccgtgc tggcgggcac cgcctgcctc ttcaccccgt 361 tGGCGCTGCC CTTAGATTAT TCCCACTACA TTTCCCTGCC CGCTGGTGTG CTGAGCCTGG 421 CCTGCTGCAC CCTCTATGGG ATCTCCTGGC AGTTTGACCC TTGCTGCAAG TACCAAGTGG 481 AGTACGACGC CTATAAACTG TCGCGCCTGC CTCTGCACAC ACTCACCTCC TCCACCCCGG 541 TGGTGCTGGT CCGGAAGGAC GACCTGCACA GAAAGAGACT GCACAACACG ATAGCACTGG 601 CCGCCCTGGT GTACTGTGTA AAGAAGATTT ACGAACTCTA TGCCGTATAG AACCCAGCTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGAATGA GCTACATCCA CTGTATTGTG ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt