Construct: ORF TRCN0000487774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021308.1_s317c1
- DNA Barcode:
- TTGGACAAGTGATCATTCTAACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ATF6B (1388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1388 | ATF6B | activating transcription fa... | NM_004381.5 | 42.9% | 39.6% | (many diffs) |
| 2 | human | 1388 | ATF6B | activating transcription fa... | NM_001136153.2 | 42.5% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1026
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggag ctgatgctgc tcagcgagat tgctgacccg acgcgtttct 121 tcaccgacaa cctgcttagc ccggaggact ggggtctgca gaacagcacc ttgtattctg 181 gcctagatga agtggccgag gagcagacgc agctcttccg ttgcccggag caggatgtcc 241 cgtttgacgg cagctccctg gacgtgggga tggatgtcag cccctctgag cccccatggg 301 aactcctgcc gatcttccca gatcttcagg tgaagtctga gccatcttcc ccctgctctt 361 cctcctccct cagctccgag tcatcgcgtc tctccacaga gccatccagc gaggctcttg 421 gggtagggga ggtgctccat gtgaagacag agtccttggc acccccactg tgtctcctgg 481 gagatgaccc aacatcctca tttgaaaccg tccagatcaa cgttatcccc acctctgatg 541 attcctcaga tgtccagacc aagatagaac ctgtctctcc atgttcttcc gtcaactctg 601 aggcctccct gctctcggcc gactcctcca gccaggcttt tataggagag gaggtcctgg 661 aagtgaagac agagtccctg tccccttcag gatgcctcct gtgggatgtc ccagccccct 721 cacttggagc tgtccagatc agcatgggcc catcccttga tggctcctca ggcaaagccc 781 tgcccacccg gaagccgcca ctgcagccca aacctgtagt gctaaccact gtcccaatgc 841 catccagagc tgtgtctccc agcaccacag tccttctgca gtccctcgtc cagccacccc 901 caggtacTGA AGAAGGAGAA AAGGGCCGGG CATGGTGGCT CACGCCGGTA ATCCCAGCAC 961 TTTGGGAGGC TGAGGCGGGC GAATCACCTG AGGTCAGAAG TTTAAGACCA GCCTGGCCAA 1021 CGTGGTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTGGAC AAGTGATCAT TCTAACCTAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt