Construct: ORF TRCN0000487796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021733.1_s317c1
- DNA Barcode:
- AACTCTCTCTATTGTTATCCCTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EPHB3 (2049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487796
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2049 | EPHB3 | EPH receptor B3 | NM_004443.4 | 39.4% | 39.4% | 1_1812del |
| 2 | mouse | 13845 | Ephb3 | Eph receptor B3 | NM_010143.1 | 35.5% | 39.1% | (many diffs) |
| 3 | mouse | 13845 | Ephb3 | Eph receptor B3 | XM_006521788.1 | 33.6% | 37% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 1284
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctacac ggagaagctg cagcagtaca ttgctcctgg aatgaaggtt tatattgacc 121 cttttaccta cgaggaccct aatgaggctg ttcgggagtt tgccaaggag atcgacgtgt 181 cctgcgtcaa gatcgaggag gtgatcggag ctggggaatt tggggaagtg tgccgtggtc 241 gactgaaaca gcctggccgc cgagaggtgt ttgtggccat caagacgctg aaggtgggct 301 acaccgagag gcagcggcgg gacttcctaa gcgaggcctc catcatgggt cagtttgatc 361 accccaatat aatccggctc gagggcgtgg tcaccaaaag tcggccagtt atgatcctca 421 ctgagttcat ggaaaactgc gccctggact ccttcctccg gctcaacgat gggcagttca 481 cggtcatcca gctggtgggc atgttgcggg gcattgctgc cggcatgaag tacctgtccg 541 agatgaacta tgtgcaccgc gacctggctg ctcgcaacat ccttgtcaac agcaacctgg 601 tctgcaaagt ctcagacttt ggcctctccc gcttcctgga ggatgacccc tccgatccta 661 cctacaccag ttccctgggc gggaagatcc ccatccgctg gactgcccca gaggccatag 721 cctatcggaa gttcacttct gctagtgatg tctggagcta cggaattgtc atgtgggagg 781 tcatgagcta tggagagcga ccctactggg acatgagcaa ccaggatgtc atcaatgccg 841 tggagcagga ttaccggctg ccaccaccca tggactgtcc cacagcactg caccagctca 901 tgctggactg ctgggtgcgg gaccggaacc tcaggcccaa attctcccag attgtcaata 961 ccctggacaa gctcatccgc aatgctgcca gcctcaaggt cattgccagc gctcagtctg 1021 gcatgtcaca gcccctcctg GACCGCACGG TCCCAGATTA CACAACCTTC ACGACAGTTG 1081 GTGATTGGCT GGATGCCATC AAGATGGGGC GGTACAAGGA GAGCTTCGTC AGTGCGGGGT 1141 TTGCATCTTT TGACCTGGTG GCCCAGATGA CGGCAGAAGA CCTGCTCCGT ATTGGGGTCA 1201 CCCTGGCCGG CCACCAGAAG AAGATCCTGA GCAGTATCCA GGACATGCGG CTGCAGATGA 1261 ACCAGACGCT GCCTGTGCAG GTCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACTCTCT 1441 CTATTGTTAT CCCTTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt