Construct: ORF TRCN0000487877

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021755.1_s317c1
DNA Barcode:
CCGGCCGGTGCATCAGAGTATGTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SIRT1 (23411)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487877
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23411 SIRT1 sirtuin 1 NM_012238.5 99.9% 100% 1440A>G
2 human 23411 SIRT1 sirtuin 1 NM_001142498.1 60.4% 60.5% 0_1ins885;555A>G
3 human 23411 SIRT1 sirtuin 1 NM_001314049.1 59.1% 57.6% (many diffs)
4 mouse 93759 Sirt1 sirtuin 1 NM_019812.3 84.6% 85.5% (many diffs)
5 mouse 93759 Sirt1 sirtuin 1 NM_001159589.2 81.2% 81.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2310
ORF length:
2241
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggcggacgag gcggccctcg cccttcagcc cggcggctcc ccctcggcgg
121cgggggccga cagggaggcc gcgtcgtccc ccgccgggga gccgctccgc aagaggccgc
181ggagagatgg tcccggcctc gagcggagcc cgggcgagcc cggtggggcg gccccagagc
241gtgaggtgcc ggcggcggcc aggggctgcc cgggtgcggc ggcggcggcg ctgtggcggg
301aggcggaggc agaggcggcg gcggcaggcg gggagcaaga ggcccaggcg actgcggcgg
361ctggggaagg agacaatggg ccgggcctgc agggcccatc tcgggagcca ccgctggccg
421acaacttgta cgacgaagac gacgacgacg agggcgagga ggaggaagag gcggcggcgg
481cggcgattgg gtaccgagat aaccttctgt tcggtgatga aattatcact aatggttttc
541attcctgtga aagtgatgag gaggatagag cctcacatgc aagctctagt gactggactc
601caaggccacg gataggtcca tatacttttg ttcagcaaca tcttatgatt ggcacagatc
661ctcgaacaat tcttaaagat ttattgccgg aaacaatacc tccacctgag ttggatgata
721tgacactgtg gcagattgtt attaatatcc tttcagaacc accaaaaagg aaaaaaagaa
781aagatattaa tacaattgaa gatgctgtga aattactgca agagtgcaaa aaaattatag
841ttctaactgg agctggggtg tctgtttcat gtggaatacc tgacttcagg tcaagggatg
901gtatttatgc tcgccttgct gtagacttcc cagatcttcc agatcctcaa gcgatgtttg
961atattgaata tttcagaaaa gatccaagac cattcttcaa gtttgcaaag gaaatatatc
1021ctggacaatt ccagccatct ctctgtcaca aattcatagc cttgtcagat aaggaaggaa
1081aactacttcg caactatacc cagaacatag acacgctgga acaggttgcg ggaatccaaa
1141ggataattca gtgtcatggt tcctttgcaa cagcatcttg cctgatttgt aaatacaaag
1201ttgactgtga agctgtacga ggagatattt ttaatcaggt agttcctcga tgtcctaggt
1261gcccagctga tgaaccgctt gctatcatga aaccagagat tgtgtttttt ggtgaaaatt
1321taccagaaca gtttcataga gccatgaagt atgacaaaga tgaagttgac ctcctcattg
1381ttattgggtc ttccctcaaa gtaagaccag tagcactaat tccaagttcc ataccccatg
1441aagtgcctca gatattaatt aatagagaac ctttgcctca tctgcatttt gatgtagagc
1501ttcttgggga ctgtgatgtc ataattaatg aattgtgtca taggttaggt ggtgaatatg
1561ccaaactttg ctgtaaccct gtaaagcttt cagaaattac tgaaaaacct ccacgaacac
1621aaaaagaatt ggcttatttg tcagagttgc cacccacacc tcttcatgtt tcagaagact
1681caagttcacc agaaagaact tcaccaccag attcttcagt gattgtcaca cttttagacc
1741aagcagctaa gagtaatgat gatttagatg tgtctgaatc aaaaggttgt atggaagaaa
1801aaccacagga agtacaaact tctaggaatg ttgaaagtat tgctgaacag atggaaaatc
1861cggatttgaa gaatgttggt tctagtactg gggagaaaaa tgaaagaact tcagtggctg
1921gaacagtgag aaaatgctgg cctaatagag tggcaaagga gcagattagt aggcggcttg
1981atggtaatca gtatctgttt ttgccaccaa atcgttacat tttccatGGC GCTGAGGTAT
2041ATTCAGACTC TGAAGATGAC GTCTTATCCT CTAGTTCTTG TGGCAGTAAC AGTGATAGTG
2101GGACATGCCA GAGTCCAAGT TTAGAAGAAC CCATGGAGGA TGAAAGTGAA ATTGAAGAAT
2161TCTACAATGG CTTAGAAGAT GAGCCTGATG TTCCAGAGAG AGCTGGAGGA GCTGGATTTG
2221GGACTGATGG AGATGATCAA GAGGCAATTA ATGAAGCTAT ATCTGTGAAA CAGGAAGTAA
2281CAGACATGAA CTATCCATCA AACAAATCAT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG
2341ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2401GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC
2461GGCCGGTGCA TCAGAGTATG TAACGCGTTA AGTCgacaat caacctctgg attacaaaat
2521ttgtgaaaga tt

Download FASTA (ORF) (Full)

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