Construct: ORF TRCN0000487918
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021246.1_s317c1
- DNA Barcode:
- TCACTTTTATTCCTAACGGCGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RHOF (54509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487918
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54509 | RHOF | ras homolog family member F... | NM_019034.3 | 76.8% | 75.3% | (many diffs) |
| 2 | mouse | 23912 | Rhof | ras homolog family member F... | NM_175092.3 | 68.8% | 66.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 582
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgcc cccggggccc tggcccagac cgccgccccc ggtccgggca 121 ggaaggagct gaagatcgtg atcgtgggcg acggcggctg cggcaagacc tcgctgctca 181 tggtgtacag ccagggctcc ttccccgagc actacgcccc atcggtgttc gagaagtaca 241 cggccagcgt gaccgttggc agcaaggagg tgaccctgaa cctcTACGAC ACGGCCGGGC 301 AAGAAGACTA TGACCGGCTG CGGCCCCTGT CCTACCAGAA CACCCACCTC GTGCTCATCT 361 GCTATGACGT CATGAATCCC ACCAGCTACG ACAACGTCCT CATCAAGTGG TTCCCTGAGG 421 TCACGCATTT CTGCCGCGGG ATCCCCATGG TGCTCATCGG CTGCAAGACA GACCTGAGGA 481 AGGACAAGGA GCAGCTGCGG AAGCTCCGGG CCGCCCAGCT GGAGCCCATC ACCTACATGC 541 AGGTGGGCCG GGGCCAAGAC CCTGGGGCAC AGCCGTGGCT GTAGAACCCA GCTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA TCACTTTTAT TCCTAACGGC GCGTACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt