Construct: ORF TRCN0000487928
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021633.1_s317c1
- DNA Barcode:
- CTGACGTACATTCCTGGAATTACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NR0B1 (190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487928
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 190 | NR0B1 | nuclear receptor subfamily ... | NM_000475.5 | 99.9% | 100% | 498G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1482
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgggc gagaaccacc agtggcaggg cagcatcctc tacaacatgc 121 ttatgagcgc gaagcaaacg cgcgcggctc ctgaggctcc agagacgcgg ctggtggatc 181 agtgctgggg ctgttcgtgc ggcgatgagc ccggggtggg cagagagggg ctgctgggcg 241 ggcggaacgt ggcgctcctg taccgctgct gcttttgcgg taaagaccac ccacggcagg 301 gcagcatcct ctacagcatg ctgacgagcg caaagcaaac gtacgcggca ccgaaggcgc 361 ccgaggcgac gctgggtccg tgctggggct gttcgtgcgg ctctgatccc ggggtgggca 421 gagcggggct tccgggtggg cggcccgtgg cactcctgta ccgctgctgc ttttgtggtg 481 aagaccaccc gcggcagggc agcatcctct acagcttgct cactagctca aagcaaacgc 541 acgtggctcc ggcagcgccc gaggcacgac cagggggcgc gtggtgggac cgctcctact 601 tcgcgcagag gccagggggt aaagaggcgc taccaggcgg gcgggccacg gcgcttctgt 661 accgctgctg cttttgcggt gaagaccacc cgcagcaggg cagcaccctc tactgcgtgc 721 ccacgagcac aaatcaagcg caggcggctc cggaggagcg gccgagggcc ccctggtggg 781 acacctcctc tggtgcgctg cggccggtgg cgctcaagag tccacaggtg gtctgcgagg 841 cagcctcagc gggcctgttg aagacgctgc gcttcgtcaa gtacttgccc tgcttccagg 901 tgctgcccct ggaccagcag ctggtgctgg tgcgcaactg ctgggcgtcc ctgctcatgc 961 ttgagctggc ccaggaccgc ttgcagttcg agactgtgga agtctcggag cccagcatgc 1021 tgcagaagat cctcaccacc aggcggcggg agaccggggg caacgagcca ctgcccgtgc 1081 ccacgctgca gcaccatttg gcaccgccgg cggaggccag gaaggtgccc tccgcctccc 1141 aggtccaagc catcaagtgc tttctttcca aatgctggag tctgaacatc agtaccaagg 1201 agtacgccta ccTCAAGGGG ACCGTGCTCT TTAACCCGGA CGTGCCGGGC CTGCAGTGCG 1261 TGAAGTACAT TCAGGGACTC CAGTGGGGAA CTCAGCAAAT ACTCAGTGAA CACACCAGGA 1321 TGACGCACCA AGGGCCCCAT GACAGATTCA TCGAACTTAA TAGTACCCTT TTCCTGCTGA 1381 GATTCATCAA TGCCAATGTC ATTGCTGAAC TGTTCTTCAG GCCCATCATC GGCACAGTCA 1441 GCATGGATGA TATGATGCTG GAAATGCTCT GTACAAAGAT ATGAGACCCA GCTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA CTGACGTACA TTCCTGGAAT TACCACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt